Spatiotemporal Reconstruction of the Human Blastocyst by Single-Cell Gene-Expression Analysis Informs Induction of Naive Pluripotency
| Autor: | Hiromitsu Nakauchi, Vittorio Sebastiano, Renee A. Reijo Pera, Sunil Pai, Larsson Omberg, Bertha Chen, Yusuke Takahashi, Jens Durruthy-Durruthy, Gugene Kang, Mark Wossidlo |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pluripotent Stem Cells Lineage (evolution) Cellular differentiation Embryonic Development Biology General Biochemistry Genetics and Molecular Biology Mixed Function Oxygenases 03 medical and health sciences Proto-Oncogene Proteins medicine Inner cell mass Humans Cell Lineage Blastocyst Genes Developmental Molecular Biology reproductive and urinary physiology Genetics Regulation of gene expression Gene Expression Profiling Embryogenesis Endoderm Gene Expression Regulation Developmental Nuclear Proteins RNA-Binding Proteins Cell Differentiation Cell Biology Cell biology Gene expression profiling Repressor Proteins 030104 developmental biology medicine.anatomical_structure Epiblast embryonic structures Single-Cell Analysis Biomarkers Germ Layers Developmental Biology |
| Zdroj: | Developmental cell. 38(1) |
| ISSN: | 1878-1551 |
| Popis: | Human preimplantation embryo development involves complex cellular and molecular events that lead to the establishment of three cell lineages in the blastocyst: trophectoderm, primitive endoderm, and epiblast. Owing to limited resources of biological specimens, our understanding of how the earliest lineage commitments are regulated remains narrow. Here, we examined gene expression in 241 individual cells from early and late human blastocysts to delineate dynamic gene-expression changes. We distinguished all three lineages and further developed a 3D model of the inner cell mass and trophectoderm in which individual cells were mapped into distinct expression domains. We identified in silico precursors of the epiblast and primitive endoderm lineages and revealed a role for MCRS1, TET1, and THAP11 in epiblast formation and their ability to induce naive pluripotency in vitro. Our results highlight the potential of single-cell gene-expression analysis in human preimplantation development to instruct human stem cell biology. |
| Databáze: | OpenAIRE |
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