Comprehensive Flow Cytometry Profiling of the Immune System in COVID-19 Convalescent Individuals
| Autor: | Sergio Gil-Manso, Iria Miguens Blanco, Rocío López-Esteban, Diego Carbonell, Luis Andrés López-Fernández, Lori West, Rafael Correa-Rocha, Marjorie Pion |
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| Rok vydání: | 2021 |
| Předmět: |
Adult
Male T Follicular Helper Cells Health Personnel Plasma Cells Immunology Antibodies Viral Lymphocyte Activation Polymerase Chain Reaction Cohort Studies Young Adult immune dysregulation Immunology and Allergy Humans unsupervised algorithms Original Research SARS-CoV-2 flow cytometry COVID-19 Convalescence RC581-607 Middle Aged Prognosis immune system Case-Control Studies Female Immunologic diseases. Allergy Dendritic Cells Follicular |
| Zdroj: | Frontiers in Immunology Frontiers in Immunology, Vol 12 (2022) |
| ISSN: | 1664-3224 |
| Popis: | SARS-CoV-2 has infected more than 200 million people worldwide, with more than 4 million associated deaths. Although more than 80% of infected people develop asymptomatic or mild COVID-19, SARS-CoV-2 can induce a profound dysregulation of the immune system. Therefore, it is important to investigate whether clinically recovered individuals present immune sequelae. The potential presence of a long-term dysregulation of the immune system could constitute a risk factor for re-infection and the development of other pathologies. Here, we performed a deep analysis of the immune system in 35 COVID-19 recovered individuals previously infected with SARS-CoV-2 compared to 16 healthy donors, by flow cytometry. Samples from COVID-19 individuals were analysed from 12 days to 305 days post-infection. We observed that, 10 months post-infection, recovered COVID-19 patients presented alterations in the values of some T-cell, B-cell, and innate cell subsets compared to healthy controls. Moreover, we found in recovered COVID-19 individuals increased levels of circulating follicular helper type 1 (cTfh1), plasmablast/plasma cells, and follicular dendritic cells (foDC), which could indicate that the Tfh-B-foDC axis might be functional to produce specific immunoglobulins 10 months post-infection. The presence of this axis and the immune system alterations could constitute prognosis markers and could play an important role in potential re-infection or the presence of long-term symptoms in some individuals. |
| Databáze: | OpenAIRE |
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