Quantitative phase imaging with scanning holographic microscopy: an experimental assesment
| Autor: | John D. Leacock, Guy Indebetouw, Yoshitaka Tada |
|---|---|
| Přispěvatelé: | Physics |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
lcsh:Medical technology
Materials science Biomedical Engineering Phase (waves) Holography 02 engineering and technology Conjugate focal plane 01 natural sciences Sensitivity and Specificity law.invention 010309 optics Biomaterials Optics law 0103 physical sciences Microscopy Radiology Nuclear Medicine and imaging Microscopy Phase-Contrast Absorption (electromagnetic radiation) Microscopy Confocal Radiological and Ultrasound Technology business.industry Phantoms Imaging Research Detector Reproducibility of Results General Medicine 021001 nanoscience & nanotechnology lcsh:R855-855.5 Differential interference contrast microscopy Microscopy Fluorescence Digital holographic microscopy 0210 nano-technology business |
| Zdroj: | BioMedical Engineering BioMedical Engineering OnLine, Vol 5, Iss 1, p 63 (2006) |
| ISSN: | 1475-925X |
| Popis: | This paper demonstrates experimentally how quantitative phase information can be obtained in scanning holographic microscopy. Scanning holography can operate in both coherent and incoherent modes, simultaneously if desired, with different detector geometries. A spatially integrating detector provides an incoherent hologram of the object's intensity distribution (absorption and/or fluorescence, for example), while a point detector in a conjugate plane of the pupil provides a coherent hologram of the object's complex amplitude, from which a quantitative measure of its phase distribution can be extracted. The possibility of capturing simultaneously holograms of three-dimensional specimens, leading to three-dimensional reconstructions with absorption contrast, reflectance contrast, fluorescence contrast, as was previously demonstrated, and quantitative phase contrast, as shown here for the first time, opens up new avenues for multimodal imaging in biological studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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