Cloning and Functional Characterisation of the Duplicated RDL Subunits from the Pea Aphid, Acyrthosiphon pisum
| Autor: | Silvia Garcia Del Villar, Andrew K. Jones |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Biology Catalysis gamma-Aminobutyric acid lcsh:Chemistry Inorganic Chemistry alternative splicing 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine GABA receptor Gene duplication medicine Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Gene Spectroscopy Fipronil fipronil Genetics Organic Chemistry Alternative splicing Acyrthosiphon pisum gene duplication neonicotinoid Neonicotinoid food and beverages General Medicine biology.organism_classification Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 nervous system chemistry 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | International Journal of Molecular Sciences Volume 19 Issue 8 International Journal of Molecular Sciences, Vol 19, Iss 8, p 2235 (2018) |
| ISSN: | 1422-0067 |
| Popis: | The insect GABA receptor, RDL (resistance to dieldrin), is a cys-loop ligand-gated ion channel (cysLGIC) that plays a central role in neuronal signaling, and is the target of several classes of insecticides. Many insects studied to date possess one Rdl gene however, there is evidence of two Rdls in aphids. To characterise further this insecticide target from pests that cause millions of dollars&rsquo worth of crop damage each year, we identified the complete cysLGIC gene superfamily of the pea aphid, Acyrthosiphon pisum, using BLAST analysis. This confirmed the presence of two Rdl-like genes (RDL1 and RDL2) that likely arose from a recent gene duplication. When expressed individually in Xenopus laevis oocytes, both subunits formed functional ion channels gated by GABA. Alternative splicing of RDL1 influenced the potency of GABA, and the potency of fipronil was different on the RDL1bd splice variant and RDL2. Imidacloprid and clothianidin showed no antagonistic activity on RDL1, whilst 100 &mu M thiacloprid reduced the GABA responses of RDL1 and RDL2 to 55% and 62%, respectively. It was concluded that gene duplication of Rdl may have conferred increased tolerance to natural insecticides, and played a role in the evolution of insect cysLGICs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |