Simulations of Diabetic and Non-Diabetic Peripheral Nerve Myelin Lipid Bilayers
| Autor: | Yiding Yuan, Yalun Yu, Jeffery B. Klauda |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Lipid Bilayers
010402 general chemistry 01 natural sciences chemistry.chemical_compound Myelin Phosphatidylcholine 0103 physical sciences Diabetes Mellitus Materials Chemistry medicine Animals Physical and Theoretical Chemistry Lipid bilayer Myelin Sheath Unsaturated fatty acid Phosphatidylethanolamine 010304 chemical physics Rats Sphingomyelins 0104 chemical sciences Surfaces Coatings and Films medicine.anatomical_structure chemistry Peripheral nervous system Phosphatidylcholines Biophysics lipids (amino acids peptides and proteins) Sciatic nerve Sphingomyelin |
| Zdroj: | The Journal of Physical Chemistry B. 125:6201-6213 |
| ISSN: | 1520-5207 1520-6106 |
| DOI: | 10.1021/acs.jpcb.1c01621 |
| Popis: | The multilayered myelin sheath is a critical component of both central and peripheral nervous systems, forming a protective barrier against axonal damage and facilitating the movement of nervous impulses. It is primarily composed of cholesterol (CHL1), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), sphingomyelin (SM), and galactosylceramide (GalCer) lipids. For rat sciatic nerve myelin (part of the peripheral nervous system, PNS), it has been found that cholesterol and unsaturated fatty acid contents are significantly lower in diabetic than in non-diabetic conditions. In this study, lipid compositions from experimental data are used to create four model rat sciatic nerve myelin lipid bilayers: PI-containing (non-diabetic and diabetic) and PS-containing (non-diabetic and diabetic), which were then simulated using the all-atom CHARMM36 force field. Simulation results of diabetic membranes indicate less rigid, more laterally expansive, and thinner bilayers as well as potentially reduced interactions between GalCer on opposing myelin leaflets, supporting a direct role of the cholesterol content decrease in instigating myelin deterioration and diabetic peripheral neuropathy. Compared to PI-lipids, PS-lipids were found to cause higher inter-lipid spacing and decreased order within membranes as a result of their smaller headgroup size and higher inter-lipid hydrogen bonding potential, which allow them to more frequently reside deeper in the membrane plane and produce pushing effects on other lipids. GalCer deuterium order parameters and non-diabetic headgroup-to-headgroup bilayer thicknesses were compared to experimental data, exhibiting close alignment, supporting the future usage of these models to study the PNS myelin sheath. |
| Databáze: | OpenAIRE |
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