Mitochondrial Morphology and Mitophagy in Heart Diseases: Qualitative and Quantitative Analyses Using Transmission Electron Microscopy
| Autor: | Martin E. Young, Mariame Selma Kane, John C. Chatham, Victor M. Darley-Usmar, Helen E. Collins, Jianhua Zhang, Silvio H. Litovsky |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Autophagy Cardiac ultrastructure 030204 cardiovascular system & hematology Mitochondrion Biology Mitochondrial morphology Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Transmission electron microscopy Mitophagy Mitochondrial fission |
| Zdroj: | Frontiers in aging. 2 |
| ISSN: | 2673-6217 |
| Popis: | Transmission electron microscopy (TEM) has long been an important technique, capable of high degree resolution and visualization of subcellular structures and organization. Over the last 20 years, TEM has gained popularity in the cardiovascular field to visualize changes at the nanometer scale in cardiac ultrastructure during cardiovascular development, aging, and a broad range of pathologies. Recently, the cardiovascular TEM enabled the studying of several signaling processes impacting mitochondrial function, such as mitochondrial fission/fusion, autophagy, mitophagy, lysosomal degradation, and lipophagy. The goals of this review are to provide an overview of the current usage of TEM to study cardiac ultrastructural changes; to understand how TEM aided the visualization of mitochondria, autophagy, and mitophagy under normal and cardiovascular disease conditions; and to discuss the overall advantages and disadvantages of TEM and potential future capabilities and advancements in the field. |
| Databáze: | OpenAIRE |
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