The AsaP1 Peptidase of Aeromonas salmonicida subsp. achromogenes Is a Highly Conserved Deuterolysin Metalloprotease (Family M35) and a Major Virulence Factor
| Autor: | Iris Hvanndal, Sarah E. Burr, B K Gudmundsdóttir, Helga Arnadottir, Joachim Frey, Valgerdur Andrésdóttir |
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| Rok vydání: | 2009 |
| Předmět: |
Virulence Factors
Salmo salar Virulence Aeromonas salmonicida Microbiology Virulence factor src Homology Domains Fish Diseases 03 medical and health sciences Bacterial Proteins Vibrionaceae Animals 14. Life underwater Molecular Biology DNA Primers Gene Library 030304 developmental biology Molecular Biology of Pathogens 0303 health sciences biology Strain (chemistry) 030306 microbiology Structural gene Proto-Oncogene Proteins c-crk biology.organism_classification Aeromonas Metalloproteases Peptide Hydrolases Plasmids MEROPS |
| Zdroj: | Journal of bacteriology |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.00847-08 |
| Popis: | Infections by the bacterium Aeromonas salmonicida subsp. achromogenes cause significant disease in a number of fish species. In this study, we showed that AsaP1, a toxic 19-kDa metallopeptidase produced by A. salmonicida subsp. achromogenes , belongs to the group of extracellular peptidases ( Aeromonas type) (MEROPS ID M35.003) of the deuterolysin family of zinc-dependent aspzincin endopeptidases. The structural gene of AsaP1 was sequenced and found to be highly conserved among gram-negative bacteria. An isogenic Δ asaP1 A. salmonicida subsp. achromogenes strain was constructed, and its ability to infect fish was compared with that of the wild-type (wt) strain. The Δ asaP1 strain was found to infect Arctic charr, Atlantic salmon, and Atlantic cod, but its virulence was decreased relative to that of the wt strain. The 50% lethal dose of the AsaP1 mutant was 10-fold higher in charr and 5-fold higher in salmon than that of the wt strain. The pathology induced by the AsaP1-deficient strain was also different from that of the wt strain. Furthermore, the mutant established significant bacterial colonization in all observed organs without any signs of a host response in the infected tissue. AsaP1 is therefore the first member of the M35 family that has been shown to be a bacterial virulence factor. |
| Databáze: | OpenAIRE |
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