Carnosic acid from rosemary extracts: a potential chemoprotective agent against aflatoxin B1. Anin vitro study
Autor: | Maria Clelia Guerra, Stefano Costa, Rinaldo Cervellati, Ester Speroni, Aneli Utan, Gianfranco Piva, Aldo Prandini |
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Rok vydání: | 2007 |
Předmět: |
Chemoprotective agent
Aflatoxin Aflatoxin B1 Guanine Cell Survival Pharmacology Protective Agents Toxicology medicine.disease_cause chemistry.chemical_compound Cell Line Tumor medicine Humans Mycotoxin Dose-Response Relationship Drug Plant Extracts Liver Neoplasms Carnosic acid Free Radical Scavengers Rosmarinus Oxidative Stress Dose–response relationship Biochemistry chemistry 8-Hydroxy-2'-Deoxyguanosine Polyphenol Abietanes Toxicity Hepatocytes Reactive Oxygen Species Oxidative stress DNA Damage |
Zdroj: | Journal of Applied Toxicology. 27:152-159 |
ISSN: | 1099-1263 0260-437X |
Popis: | Since oxidative stress plays an important role in the toxicity mechanism of several mycotoxins such as aflatoxin B1 (AFB1), the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Carnosic acid (CA) is the major polyphenolic compound present in rosemary plants and it can also be found in sage leaves. Its free radical scavenging properties were tested with two chemical methods. It was found that it has good free radical scavenging capacity at pH 7.4. This study also found that a 24 h pre-treatment with 10, 20 and 30 microm CA led to a clear, dose-dependent protective effect on cell toxicity, reducing cell death induced by a 24 h exposure with 10 microm AFB1, respectively, by 16% (P < 0.05), 26% (P < 0.01) and 63% (P < 0.001). It was also found that a 24 h pre-treatment with 20 and 30 microm CA achieved a reduction of ROS levels, respectively, of 146% (P < 0.001) and 173% (P < 0.001) in HepG2 cells exposed to 10 microm AFB1 for 8 h. Moreover, in cells pre-incubated with 30 microm CA for 24 h the concentration of 8-OH-deoxyguanine decreased by 57% (P < 0.001) with respect to the cells exposed for 24 h to 10 microm AFB1 alone. The results obtained with the in vitro and chemical studies support the theory that AFB1 induced oxidative stress plays an important role in the cytotoxic mechanism of this mycotoxin. Furthermore these findings suggest a starting point for developing alimentary strategies in order to counteract the damage caused by AFB1 contamination in feed and food. |
Databáze: | OpenAIRE |
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