Inhibition of hepatic neoglucogenesis and glucose-6-phosphatase by quercetin 3-O-α(2″-galloyl)rhamnoside isolated fromBauhinia megalandra leaves
| Autor: | Freddy González-Mujica, Masahisa Hasegawa, Elsa Perdomo, Omar Estrada, Norma Motta, Jeannette Mendez |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Monosaccharide Transport Proteins Flavonoid Biology Pyrophosphate Antiporters Rats Sprague-Dawley chemistry.chemical_compound Animals Enzyme Inhibitors Pharmacology chemistry.chemical_classification Pyrophosphatase Dose-Response Relationship Drug Plant Extracts Gluconeogenesis Rats Plant Leaves Enzyme Biochemistry chemistry Bauhinia Glucose-6-Phosphatase Microsomes Liver Microsome biology.protein Quercetin Glucose 6-phosphatase Phytotherapy |
| Zdroj: | Phytotherapy Research. 19:624-627 |
| ISSN: | 1099-1573 0951-418X |
| DOI: | 10.1002/ptr.1704 |
| Popis: | In intact microsomes, quercetin 3-O-α-(2″-galloyl)rhamnoside (QGR) inhibits glucose-6-phosphatase (G-6-Pase) in a concentration-dependent manner. QGR increased the G-6-Pase Km for glucose-6-phosphate without change in the Vmax. The flavonol did not change the kinetic parameters of disrupted microsomal G-6-Pase or intact or disrupted microsomal G-6-Pase pyrophosphatase (PPase) activity. This result allowed the conclusion that QGR competitively inhibits the glucose-6-phosphate (G-6-P) transporter (T1) without affecting the catalytic subunit or the phosphate/pyrophosphate transporter (T2) of the G-6-Pase system. QGR strongly inhibits the neoglucogenic capacity of rat liver slices incubated in a Krebs-Ringer bicarbonate buffer, supplemented with lactate and oleate saturated albumin. The QGR G-6-Pase inhibition might explain the decrease in the liver slice neoglucogenic capacity and, in turn, could reduce glucose levels in diabetic patients. Copyright © 2005 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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