GPR124 regulates microtubule assembly, mitotic progression, and glioblastoma cell proliferation
| Autor: | Nephi Stella, Shao En Ong, Richard S. Morrison, Cong Xu, Juan Jesus Vicente, Linda Wordeman, Allison E. Cherry |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Regulator Gene Expression Mitosis Biology Proteomics Microtubules Article Receptors G-Protein-Coupled 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Microtubule Gene expression Humans Receptor Cells Cultured Cell Proliferation Cell growth Brain Neoplasms Embryogenesis Cell Membrane Brain Middle Aged Cell biology 030104 developmental biology Neurology Female Glioblastoma Microtubule-Associated Proteins 030217 neurology & neurosurgery |
| Zdroj: | Glia |
| ISSN: | 1098-1136 |
| Popis: | GPR124 is involved in embryonic development and remains expressed by select organs. The importance of GPR124 during development suggests that its aberrant expression might participate in tumor growth. Here we show that both increases and decreases in GPR124 expression in glioblastoma cells reduce cell proliferation by differentially altering the duration mitotic progression. Using mass spectrometry-based proteomics, we discovered that GPR124 interacts with ch-TOG, a known regulator of both microtubule (MT)-plus-end assembly and mitotic progression. Accordingly, changes in GPR124 expression and ch-TOG similarly affect MT assembly measured by real-time microscopy in cells. Our study describes a novel molecular interaction involving GPR124 and ch-TOG at the plasma membrane that controls glioblastoma cell proliferation by modifying MT assembly rates and controlling the progression of distinct phases of mitosis. |
| Databáze: | OpenAIRE |
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