Transcription factor TBX18 promotes adult rat bone mesenchymal stem cell differentiation to biological pacemaker cells

Autor: Mei Yang, Ge-Ge Zhang, Yanhong Tang, Bingjie Ye, Congxin Huang, Yanjun Li, Le Li
Rok vydání: 2017
Předmět:
0301 basic medicine
Potassium Channels
Biological pacemaker
Cellular differentiation
Cell
Bone Marrow Cells
Biology
transcription factor T-Box protein 18
Adenoviridae
03 medical and health sciences
stomatognathic system
Biological Clocks
Osteogenesis
Cancer stem cell
Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
Genetics
medicine
Animals
Humans
bone mesenchymal stem cells
Sinoatrial Node
Myocardium
Troponin I
Mesenchymal stem cell
reprogramming
Gene Expression Regulation
Developmental
biological pacemaker
Cell Differentiation
Mesenchymal Stem Cells
Articles
differentiation
General Medicine
Actins
Rats
030104 developmental biology
medicine.anatomical_structure
Connexin 43
Cancer research
Myeloid-derived Suppressor Cell
Mesenchymal stem cell differentiation
Stem cell
T-Box Domain Proteins
Zdroj: International Journal of Molecular Medicine
ISSN: 1791-244X
1107-3756
Popis: Bone mesenchymal stem cells (BMSCs) are currently considered the optimal stem cells for biological pacemaker cell transformation. The cardiac-specific transcription factor T-Box protein 18 (TBX18) is essential for sinoatrial node (SAN) formation, particularly formation of the head region that generates the electrical impulses that induce heart contraction. The present study aimed to confirm the effects of TBX18 on biological pace-maker differentiation of rat BMSCs. Flow cytometry was used to identify the surface markers of BMSCs, in order to acquire pure mesenchymal stem cells. Subsequently, BMSCs were transduced with TBX18 or green fluorescent protein adenovirus vectors. The effects of TBX18 were evaluated using SAN-specific makers including TBX18, α-actin, cardiac troponin I, hyperpolarization-activated cyclic nucleotide-gated channel 4 and connexin 43 by reverse transcription-quantitative polymerase chain reaction, western blotting and immunofluorescence. The findings demonstrated that direct conversion of BMSCs to biological pacemaker cells via TBX18 is a feasible method in the field of cardiology.
Databáze: OpenAIRE
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