Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
| Autor: | Pieter van Dillewijn, Jesús Muñoz-Rojas, Jesús de la Torre, Laura Abisaí Pazos-Rojas, Verónica Quintero-Hernández, Antonino Baez, Yolanda Elizabeth Morales-García, Lilia I. López-Lara, Lesther Emanuel López-Cruz |
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| Přispěvatelé: | Benemérita Universidad Autónoma de Puebla, Consejo Nacional de Ciencia y Tecnología (México) |
| Rok vydání: | 2020 |
| Předmět: |
Siderophore
Survival Resuscitation Phenylalanine Microbiology Applied Microbiology and Biotechnology Viable but nonculturable Quintero-Hernández VBNC 03 medical and health sciences TonB-dependent receptor 030304 developmental biology 0303 health sciences biology 030306 microbiology Catabolism Chemistry López-Cruz L.E Membrane transport biology.organism_classification QR1-502 Pseudomonas putida Morales-García Y.E bacteria Desiccation Pazos-Rojas L.A Bacteria |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Annals of Microbiology, Vol 70, Iss 1, Pp 1-11 (2020) |
| Popis: | Purpose: Pseudomonas putida KT2440 is a desiccation-sensitive bacterium that loses culturability after 15 days of air desiccation. We have previously shown that P. putida KT2440 can develop a viable but nonculturable (VBNC) state after being exposed to desiccation stress and eventually recover when desiccated cells are rehydrated for at least 24 h. Methods: To determine which genes of transport, oxidation-reduction, and transcription processes could be involved in the return of P. putida KT2440 to the culturable state, a transcriptome analysis was carried out comparing the gene expression of non-desiccated samples with samples subjected to desiccation followed by 20 min of rehydration or desiccation followed by 24 h of rehydration. Results: Desiccation stress triggered a VBNC state of P. putida. The major response was detected after 24 h of rehydration with 148 upregulated and 42 downregulated genes. During the VBNC state, P. putida activated transmembrane transport processes like that of siderophores through a TonB-dependent transporter and putative polyhydric alcohol transport systems. Prolonged rehydration with distilled water resuscitated P. putida KT2440 cells activating the catabolism of phenylalanine/tyrosine to provide energy and carbon for ubiquinone biosynthesis while maintaining a reduced protein synthesis. On the other hand, the interruption of the TonB-dependent receptor gene (PP_1446) increased desiccation survival of the mutant strain. Conclusion: The activation of the iron transport system (TonB-dependent siderophore receptor) and alcohol transport can be helping the VBNC state of P. putida. Activation of catabolism of phenylalanine/tyrosine and reduced protein synthesis was needed for resuscitation from the VBNC state. Funding was provided by the intramural program of the Vicerrectoria de Investigación y Estudios de Posgrado, BUAP through grants PV16-ID00450, PV17-ID00337. Lilia Isela Lopez Lara received a scholarship (number 273274) from the National Council of Science and Technology (CONACyT). |
| Databáze: | OpenAIRE |
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