Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species
| Autor: | Francisca Aliny Nunes Silva, André Gonzaga dos Santos, Érica de Oliveira Lopes, Guilherme Julião Zocolo, Margarete Teresa Gottardo de Almeida, Tigressa Helena Rodrigues Soares, Taís Maria Bauab, E. M. Castilho, Fernando Rogério Pavan, Luciani Gaspar de Toledo, Matheus Aparecido dos Santos Ramos, Larissa Spósito |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antifungal Agents gas chromatography 030106 microbiology Hyphae Cymbopogon nardus essential oil Candida antifungal activity Microbial Sensitivity Tests Catalysis Article Gas Chromatography-Mass Spectrometry law.invention Microbiology lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration Inhibitory Concentration 50 law Oils Volatile Physical and Theoretical Chemistry Cymbopogon Candida albicans lcsh:QH301-705.5 Molecular Biology Spectroscopy Essential oil Citronellol biology Organic Chemistry General Medicine biology.organism_classification Corpus albicans Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry Citronellal Geraniol |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences; Volume 17; Issue 8; Pages: 1252 International Journal of Molecular Sciences, Vol 17, Iss 8, p 1252 (2016) |
| ISSN: | 1422-0067 |
| Popis: | Background: The incidence of fungal infections, especially those caused by Candida yeasts, has increased over the last two decades. However, the indicated therapy for fungal control has limitations. Hence, medicinal plants have emerged as an alternative in the search for new antifungal agents as they present compounds, such as essential oils, with important biological effects. Published data demonstrate important pharmacological properties of the essential oil of Cymbopogon nardus (L.) Rendle; these include anti-tumor, anti-nociceptive, and antibacterial activities, and so an investigation of this compound against pathogenic fungi is interesting. Objective: The aim of this study was to evaluate the chemical composition and biological potential of essential oil (EO) obtained from the leaves of C. nardus focusing on its antifungal profile against Candida species. Methods: The EO was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Testing of the antifungal potential against standard and clinical strains was performed by determining the minimal inhibitory concentration (MIC), time-kill, inhibition of Candida albicans hyphae growth, and inhibition of mature biofilms. Additionally, the cytotoxicity was investigated by the IC50 against HepG-2 (hepatic) and MRC-5 (fibroblast) cell lines. Results: According to the chemical analysis, the main compounds of the EO were the oxygen-containing monoterpenes: citronellal, geranial, geraniol, citronellol, and neral. The results showed important antifungal potential for all strains tested with MIC values ranging from 250 to 1000 μg/mL, except for two clinical isolates of C. tropicalis (MIC > 1000 μg/mL). The time-kill assay showed that the EO inhibited the growth of the yeast and inhibited hyphal formation of C. albicans strains at concentrations ranging from 15.8 to 1000 μg/mL. Inhibition of mature biofilms of strains of C. albicans, C. krusei and C. parapsilosis occurred at a concentration of 10× MIC. The values of the IC50 for the EO were 96.6 μg/mL (HepG-2) and 33.1 μg/mL (MRC-5). Conclusion: As a major virulence mechanism is attributed to these types of infections, the EO is a promising compound to inhibit Candida species, especially considering its action against biofilm. |
| Databáze: | OpenAIRE |
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