Global gene expression analysis of cranial neural tubes in embryos of diabetic mice
| Autor: | Eng-Ang Ling, Boran Jiang, Jayapal Manikandan, S. Thameem Dheen, S Dinesh Kumar, Wan Ting Loh, Samuel Sam Wah Tay |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Neural Tube Proliferation index Apoptosis Nerve Tissue Proteins In situ hybridization Biology Cerebral Ventricles Diabetes Mellitus Experimental Diabetes Complications Andrology Mice Cellular and Molecular Neuroscience Pregnancy medicine Animals Cell Lineage Neural Tube Defects Body Patterning Cell Proliferation Neurons TUNEL assay Stem Cells Neurogenesis Neural tube Brain Gene Expression Regulation Developmental Cell Differentiation Molecular biology Gene expression profiling Neuroepithelial cell Disease Models Animal medicine.anatomical_structure Terminal deoxynucleotidyl transferase Female |
| Zdroj: | Journal of Neuroscience Research. 86:3481-3493 |
| ISSN: | 1097-4547 0360-4012 |
| Popis: | Maternal diabetes causes congenital malformations in various organs including the neural tube in fetuses. In this study, we have analyzed the differential gene expression profiling in the cranial neural tube of embryos from diabetic and control mice by using the oligonucleotide microarray. Expression patterns of genes and proteins that are differentially expressed in the cranial neural tube were further examined by the real-time reverse transcriptase-polymerase chain reaction, in situ hybridization, and immunohistochemistry. Proliferation index and apoptosis were examined by BrdU (5-bromo-2-deoxyuridine) labeling and TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling) assay, respectively. Embryos (E11.5) of diabetic pregnancies displayed distortion in neuroepithelia of the cranial neural tube. Microarray analysis revealed that a total of 390 genes exhibited more than twofold changes in expression level in the cranial neural tube of embryos from diabetic mice. Several genes involving apoptosis, proliferation, migration, and differentiation of neurons in the cranial neural tube were differentially expressed in embryos of diabetic pregnancy. In addition, maternal diabetes perturbed the development of choroid plexus and ventricular systems and reduced the production of proteins such as Ttr and Igf2 in the developing brain, indicating that these changes could impair the survival and proliferation of neuroepithelial cells and neurogenesis in embryos of diabetic mice. It is concluded that altered expression of a variety of genes involved in brain development is associated with cranial neural tube dysmorphogenesis that may subsequently contribute to intellectual impairment of the offspring of a diabetic mother. |
| Databáze: | OpenAIRE |
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