TNF-α Regulated Endometrial Stroma Secretome Promotes Trophoblast Invasion
Autor: | Gil Mor, Anthony Maxwell, Patrick Stelzl, Shannon Whirledge, Ai-Hua Liao, Dana N. Joseph, Yuan You, Nava Dekel, Paulomi Aldo |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Stromal cell
medicine.medical_treatment Immunology Inflammation Biology Endometrium migration Cell Line Stroma Cell Movement Paracrine Communication Cell Adhesion cytokine medicine Humans Immunology and Allergy embryo implantation Blastocyst reproductive and urinary physiology Original Research Secretory Pathway Tumor Necrosis Factor-alpha Interleukin-17 Trophoblast Cell Differentiation RC581-607 invasion trophoblast In vitro Trophoblasts Cell biology medicine.anatomical_structure Cytokine Receptors Tumor Necrosis Factor Type I inflammation TNF-α embryonic structures Female Stromal Cells medicine.symptom Immunologic diseases. Allergy Signal Transduction |
Zdroj: | Frontiers in Immunology, Vol 12 (2021) Frontiers in Immunology |
ISSN: | 1664-3224 |
Popis: | Successful implantation requires the coordinated migration and invasion of trophoblast cells from out of the blastocyst and into the endometrium. This process relies on signals produced by cells in the maternal endometrium. However, the relative contribution of stroma cells remains unclear. The study of human implantation has major technical limitations, therefore the need of in vitro models to elucidate the molecular mechanisms. Using a recently described 3D in vitro models we evaluated the interaction between trophoblasts and human endometrial stroma cells (hESC), we assessed the process of trophoblast migration and invasion in the presence of stroma derived factors. We demonstrate that hESC promotes trophoblast invasion through the generation of an inflammatory environment modulated by TNF-α. We also show the role of stromal derived IL-17 as a promoter of trophoblast migration through the induction of essential genes that confer invasive capacity to cells of the trophectoderm. In conclusion, we describe the characterization of a cellular inflammatory network that may be important for blastocyst implantation. Our findings provide a new insight into the complexity of the implantation process and reveal the importance of inflammation for embryo implantation. |
Databáze: | OpenAIRE |
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