Comparative transcriptomics of choroid plexus in Alzheimer’s disease, frontotemporal dementia and Huntington’s disease: implications for CSF homeostasis

Autor: Alexei A. Podtelezhnikov, Conrad E. Johanson, Luiz M Camargo, Andrew Baird, John E. Donahue, David J. Stone, Gerald D. Silverberg, Eva M. Finney, Brian P. Eliceiri, Miles C. Miller, Lisan Parker, Elena Nikonova, Edward G. Stopa, Tara Torabi, Keith Q. Tanis, Ajay Verma
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Cadherin-mediated adhesion
CSF homocysteine
Gene Expression
lcsh:RC346-429
Transcriptome
0302 clinical medicine
Gene expression
Homeostasis
Choroid plexus transcriptome
Aged
80 and over
Neurodegeneration
General Medicine
Middle Aged
Huntington Disease
Neurology
Frontotemporal Dementia
Female
Choroid plexus
Choroid plexus methionine
Frontotemporal dementia
Adult
LRP-1
Peroxisome-proliferator-activated receptor (PPAR)
Biology
Janus kinase/signal transducers and activators of transcription (JAK-STAT)
03 medical and health sciences
Cellular and Molecular Neuroscience
Developmental Neuroscience
Huntington's disease
Alzheimer Disease
medicine
Humans
Dementia
Mechanistic target of rapamycin (mTOR)
PI3K/AKT/mTOR pathway
lcsh:Neurology. Diseases of the nervous system
Aged
Research
Neuroimmune CSF regulation
Microarray Analysis
medicine.disease
Blood–CSF barrier inflammatome
030104 developmental biology
Choroid Plexus
Cancer research
Vascular endothelial growth factor
030217 neurology & neurosurgery
Zdroj: Fluids and Barriers of the CNS, Vol 15, Iss 1, Pp 1-10 (2018)
Fluids and Barriers of the CNS
ISSN: 2045-8118
DOI: 10.1186/s12987-018-0102-9
Popis: Background In Alzheimer’s disease, there are striking changes in CSF composition that relate to altered choroid plexus (CP) function. Studying CP tissue gene expression at the blood–cerebrospinal fluid barrier could provide further insight into the epithelial and stromal responses to neurodegenerative disease states. Methods Transcriptome-wide Affymetrix microarrays were used to determine disease-related changes in gene expression in human CP. RNA from post-mortem samples of the entire lateral ventricular choroid plexus was extracted from 6 healthy controls (Ctrl), 7 patients with advanced (Braak and Braak stage III–VI) Alzheimer’s disease (AD), 4 with frontotemporal dementia (FTD) and 3 with Huntington’s disease (HuD). Statistics and agglomerative clustering were accomplished with MathWorks, MatLab; and gene set annotations by comparing input sets to GeneGo (http://www.genego.com) and Ingenuity (http://www.ingenuity.com) pathway sets. Bonferroni-corrected hypergeometric p-values of
Databáze: OpenAIRE
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