The Coding and Small Non-coding Hippocampal Synaptic RNAome
| Autor: | Andre Fischer, Dennis M. Krüger, Sarah Köster, Tea Berulava, Gerrit Brehm, Rezaul Islam, Robert Epple |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male RNA Untranslated mRNA Microfluidics Neuroscience (miscellaneous) Hippocampus metabolism [Hippocampus] Hippocampal formation Biology snoRNA Article Synapse metabolism [RNA Untranslated] 03 medical and health sciences Cellular and Molecular Neuroscience genetics [RNA Messenger] 0302 clinical medicine lncRNA Postsynaptic potential ddc:570 Animals genetics [MicroRNAs] RNA Messenger Small nucleolar RNA metabolism [Synaptosomes] genetics [RNA Untranslated] Neurons Messenger RNA microRNA RNA Correction Translation (biology) RNA sequencing metabolism [Synapses] Mice Inbred C57BL MicroRNAs 030104 developmental biology Neurology metabolism [Neurons] Synapses Gene expression Neuroscience 030217 neurology & neurosurgery Synaptosomes |
| Zdroj: | Mol Neurobiol Molecular neurobiology 58(6), 2940-2953 (2021). doi:10.1007/s12035-021-02296-y Molecular Neurobiology |
| ISSN: | 1559-1182 |
| DOI: | 10.1007/s12035-021-02296-y |
| Popis: | Neurons are highly compartmentalized cells that depend on local protein synthesis. Messenger RNAs (mRNAs) have thus been detected in neuronal dendrites, and more recently in the pre- and postsynaptic compartments as well. Other RNA species such as microRNAs have also been described at synapses where they are believed to control mRNA availability for local translation. A combined dataset analyzing the synaptic coding and non-coding RNAome via next-generation sequencing approaches is, however, still lacking. Here, we isolate synaptosomes from the hippocampus of young wild-type mice and provide the coding and non-coding synaptic RNAome. These data are complemented by a novel approach for analyzing the synaptic RNAome from primary hippocampal neurons grown in microfluidic chambers. Our data show that synaptic microRNAs control almost the entire synaptic mRNAome, and we identified several hub microRNAs. By combining the in vivo synaptosomal data with our novel microfluidic chamber system, our findings also support the hypothesis that part of the synaptic microRNAome may be supplied to neurons via astrocytes. Moreover, the microfluidic system is suitable for studying the dynamics of the synaptic RNAome in response to stimulation. In conclusion, our data provide a valuable resource and point to several important targets for further research. Supplementary Information The online version contains supplementary material available at 10.1007/s12035-021-02296-y. |
| Databáze: | OpenAIRE |
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