Agr2-interacting Prod1-like protein Tfp4 from Xenopus laevis is necessary for early forebrain and eye development as well as for the tadpole appendage regeneration
| Autor: | Elena A. Solovieva, Alexey M. Nesterenko, Daria D. Korotkova, Eugeny E. Orlov, Andrey V. Bayramov, Anastasiya S. Ivanova, Maria B. Tereshina, Natalia Y. Martynova, Andrey G. Zaraisky, Fedor M. Eroshkin, Elena A. Parshina |
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| Rok vydání: | 2018 |
| Předmět: |
Organogenesis
Xenopus AGR2 Embryonic Development Biology Xenopus Proteins 03 medical and health sciences Xenopus laevis 0302 clinical medicine Endocrinology Thioredoxins Genetics Animals Regeneration 030304 developmental biology 0303 health sciences Arginase Regeneration (biology) Gene Expression Regulation Developmental Embryo Extremities Cell Biology biology.organism_classification Cell biology Secretory protein Larva Forebrain Eye development Thioredoxin Carrier Proteins 030217 neurology & neurosurgery Protein Binding |
| Zdroj: | Genesis (New York, N.Y. : 2000). 57(5) |
| ISSN: | 1526-968X |
| Popis: | The Agr family genes, Ag1, Agr2, and Agr3, encode for the thioredoxin domain containing secreted proteins and are specific only for vertebrates. These proteins are attracting increasing attention due to their involvement in many physiological and pathological processes, including exocrine secretion, cancer, regeneration of the body appendages, and the early brain development. At the same time, the mode by which Agrs regulate intracellular processes are poorly understood. Despite that the receptor to Agr2, the membrane anchored protein Prod1, has been firstly discovered in Urodeles, and it has been shown to interact with Agr2 in the regenerating limb, no functional homologs of Prod1 were identified in other vertebrates. This raises the question of the mechanisms by which Agrs can regulate regeneration in other lower vertebrates. Recently, we have identified that Tfp4 (three-fingers Protein 4), the structural and functional homolog of Prod1 in Anurans, interacts with Agr2 in Xenopus laevis embryos. In the present work we show by several methods that the activity of Tfp4 is essential for the tadpole tail regeneration as well as for the early eye and forebrain development during embryogenesis. These data show for the first time the common molecular mechanism of regeneration regulation in amphibians by interaction of Prod1 and Agr2 proteins. |
| Databáze: | OpenAIRE |
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