Corrigendum : FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons
| Autor: | Eduardo Soriano, Javier DeFelipe, Cátia M. Teixeira, Angel Merchán-Pérez, Isabel Fernaud, Fausto Ulloa, Albert Martínez, Carlos Lois, Carles Bosch, Joan X. Comella, Esther Pérez-Martínez, Nuria Masachs |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Dendritic spine Neuroscience (miscellaneous) Adult neurogenesis Dendritic spines Green fluorescent protein 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Electron microscopy electron microscopy 3D-reconstruction Chemistry 3D reconstruction Neurogenesis Correction dendritic spines Cell biology adult neurogenesis 030104 developmental biology FIB/SEM Synapses synapses Anatomy 030217 neurology & neurosurgery Neuroscience |
| Zdroj: | Frontiers in Neuroanatomy |
| Popis: | The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion beam/scanning electron microscopy (FIB/SEM) allows efficient, complete, and automatic 3D reconstruction of identified dendrites, including their spines and synapses, from GFP/DAB-labeled neurons, with a resolution comparable to that of TEM. We applied this technology to analyze the synaptogenesis of labeled adult-generated granule cells (GCs) in mice. 3D reconstruction of dendritic spines in GCs aged 3-4 and 8-9 weeks revealed two different stages of dendritic spine development and unexpected features of synapse formation, including vacant and branched dendritic spines and presynaptic terminals establishing synapses with up to 10 dendritic spines. Given the reliability, efficiency, and high resolution of FIB/SEM technology and the wide use of DAB in conventional EM, we consider FIB/SEM fundamental for the detailed characterization of identified synaptic contacts in neurons in a high-throughput manner. |
| Databáze: | OpenAIRE |
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