Expression of human lymphotoxin in Namalwa KJM-1 cells adapted to serum-free medium
| Autor: | Tamio Mizukami, Nobuo Fujiyoshi, Seiga Itoh, Hiromasa Miyaji, Nahoko Harada, Seiji Sato |
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| Rok vydání: | 1990 |
| Předmět: |
Genetic Vectors
Clinical Biochemistry Biomedical Engineering Clone (cell biology) Bioengineering Simian virus 40 Biology Transfection Virus Cell Line law.invention Electricity law Complementary DNA Humans Lymphotoxin-alpha Gene Expression vector Electroporation Cell Biology Molecular biology Recombinant Proteins Culture Media Lymphotoxin Recombinant DNA Biotechnology |
| Zdroj: | Cytotechnology. 4:39-43 |
| ISSN: | 1573-0778 0920-9069 |
| DOI: | 10.1007/bf00148809 |
| Popis: | A Namalwa cell line, KJM-1, which was adapted to serum-free medium is thought to be a good host cell line for recombinant DNA technology. We previously reported the expression of human beta-interferon (beta-IFN) in Namalwa KJM-1 (Miyaji, 1989a). The utility of Namalwa KJM-1 for expression of foreign genes was further examined. As a target gene to be expressed, human lymphotoxin (hLT) cDNA was used. It was engineered for expression in Namalwa KJM-1 using a simian virus 40 (SV40)-based expression vector pAGE107 (Miyaji, 1989a). It contains all components necessary for the expression of cDNA in mammalian cells. The expression vector was introduced into Namalwa KJM-1 by electroporation. Among the transformants, clone 7 was further examined for the expression of hLT in serum-free medium. The production level of hLT was augmented with the increase of the cell density. Thus it was further indicated that Namalwa KJM-1 is useful for production of foreign gene products. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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