Oral microbiota and salivary levels of oral pathogens in gastro‐intestinal diseases: Current knowledge and exploratory study
| Autor: | Antonietta Gerarda Gravina, Giovanna Donnarumma, Gianna Dipalma, Stefania Lama, Rosario Serpico, Alessandra Fusco, Alessandro Federico, Annalisa Itro, Francesco Inchingolo, Paola Stiuso, Angelo Itro, Maria Contaldo |
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| Přispěvatelé: | Contaldo, M., Fusco, A., Stiuso, P., Lama, S., Gravina, A. G., Itro, A., Federico, A., Dipalma, G., Inchingolo, F., Serpico, R., Donnarumma, G. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Candida albican RT‐PCR QH301-705.5 RT-PCR Chronic gastritis Review Porphyromonas gingivali Porphyromonas gingivalis Microbiology oral dysbiosis 03 medical and health sciences 0302 clinical medicine Virology Candida albicans medicine Microbiome Biology (General) biology Fusobacterium nucleatum business.industry Fusobacteria 030206 dentistry Salivary markers biology.organism_classification medicine.disease Corpus albicans stomatognathic diseases Chronic gastriti 030104 developmental biology Oral microbiota chronic gastritis Immunology Oral Microbiome business Oral dysbiosi |
| Zdroj: | Microorganisms Microorganisms, Vol 9, Iss 1064, p 1064 (2021) |
| Popis: | Various bi-directional associations exist between oral health and gastro-intestinal diseases. The oral microbiome plays a role in the gastro-intestinal carcinogenesis and fusobacteria are the most investigated bacteria involved. This paper aims to review the current knowledge and report the preliminary data on salivary levels of Fusobacterium nucleatum, Porphyromonas gingivalis and Candida albicans in subjects with different gastro-intestinal conditions or pathologies, in order to determine any differences. The null hypothesis was “subjects with different gastro-intestinal diseases do not show significant differences in the composition of the oral microbiota”. Twenty-one subjects undergoing esophagastroduodenoscopy or colonscopy were recruited. For each subject, a salivary sample was collected before the endoscopy procedure, immediately stored at −20 °C and subsequently used for genomic bacterial DNA extraction by real-time PCR. Low levels of F. nucleatum and P. gingivalis were peculiar in the oral microbiota in subjects affected by Helicobater pylori-negative chronic gastritis without cancerization and future studies will elucidate this association. The level of C. albicans did not statistically differ among groups. This preliminary study could be used in the future, following further investigation, as a non-invasive method for the search of gastrointestinal diseases and associated markers. |
| Databáze: | OpenAIRE |
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