Microencapsulated olfactory ensheathing-cell transplantation reduces pain in rats by inhibiting P2X4 receptor overexpression in the dorsal root ganglion
| Autor: | Yuguo Liu, Wen-jun Zhang, Bao-lin Yang, Zeng-xu Liu, Qing Yu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Alginates Cell Transplantation Drug Compounding Gene Expression 03 medical and health sciences Random Allocation 0302 clinical medicine Dorsal root ganglion Internal medicine Ganglia Spinal medicine Animals Receptor Cells Cultured business.industry General Neuroscience Sciatic nerve injury medicine.disease Olfactory Bulb Olfactory bulb Rats Transplantation Blot 030104 developmental biology Endocrinology medicine.anatomical_structure Neuropathic pain Female Olfactory ensheathing glia business Receptors Purinergic P2X4 030217 neurology & neurosurgery |
| Zdroj: | Neuroreport. 30(2) |
| ISSN: | 1473-558X |
| Popis: | The aim of this study was to determine the role of microencapsulated olfactory ensheathing-cell transplantation (MC-OEC) in rats in which pain was induced by sciatic nerve injury, and its relationship with the expression level of the P2X4 receptor in the dorsal root ganglion. Olfactory bulb tissues of healthy Sprague-Dawley rats were collected to culture olfactory ensheathing cells using differential attachment methods. Ninety-six healthy Sprague-Dawley rats were randomly assigned to the sham, chronic constriction injury (CCI), olfactory ensheathing cell (OEC), and MC-OEC groups. Mechanical paw withdrawal thresholds were measured 7 and 14 days after surgery. The expression of P2X4 receptor genes in the L4-5 dorsal root ganglion was detected by reverse transcriptase polymerase chain reaction, fluorescence in-situ hybridization, and western blotting. Seven and 14 days after the surgery, the mechanical paw withdrawal thresholds of rats in the MC-OEC, OEC, and CCI groups were decreased compared with the sham group. The expression level of the P2X4 receptor in the L4-5 dorsal root ganglion in CCI, OEC, and MC-OEC groups was increased compared with the sham group. All differences between groups were statistically significant. Transplantation of OEC and MC-OEC can reduce neuropathic pain and inhibit the overexpression of the P2X4 receptor in the L4-5 dorsal root ganglion. The transplantation of MC-OEC was more effective in the MC-OEC group than in the OEC group. |
| Databáze: | OpenAIRE |
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