Immediate remote ischemic postconditioning after hypoxia ischemia in piglets protects cerebral white matter but not grey matter

Autor: Xavier Golay, Aaron Oliver-Taylor, I Fierens, M Ezzati, Pierre Gressens, Daniel Alonso-Alconada, Kate Bennett, Nicola J. Robertson, Derek M. Yellon, Alan Bainbridge, Ilias Tachtsidis, Bobbi Fleiss, Kevin D. Broad, Jamshid Rostami, Sophie Lebon, K Jane Hassell, Mariya Hristova, Derek J. Hausenloy, Eridan Rocha-Ferreira, G Kawano
Jazyk: angličtina
Rok vydání: 2015
Předmět:
0301 basic medicine
Internal capsule
Magnetic Resonance Spectroscopy
Swine
Gene Expression
hypoxia–ischemia
Corpus callosum
remote ischemic postconditioning
0302 clinical medicine
Adenosine Triphosphate
KATP Channels
Medicine
Gray Matter
Ischemic Postconditioning
Brain Mapping
neonatal encephalopathy
Electroencephalography
Receptor
Endothelin A

Immunohistochemistry
White Matter
3. Good health
medicine.anatomical_structure
Neurology
Lower Extremity
Anesthesia
Hypoxia-Ischemia
Brain

Cardiology
neuroprotection
Cardiology and Cardiovascular Medicine
medicine.medical_specialty
Ischemia
Grey matter
Neuroprotection
White matter
03 medical and health sciences
Internal medicine
Animals
Lactic Acid
Aspartic Acid
business.industry
Neonatal encephalopathy
Birth asphyxia
Original Articles
medicine.disease
Disease Models
Animal

030104 developmental biology
Animals
Newborn

Neurology (clinical)
business
030217 neurology & neurosurgery
Biomarkers
Zdroj: Journal of Cerebral Blood Flow & Metabolism
Ezzati, M, Bainbridge, A, Broad, K D, Kawano, G, Oliver-Taylor, A, Rocha-Ferreira, E, Alonso-Alconada, D, Fierens, I, Rostami, J, Jane Hassell, K, Tachtsidis, I, Gressens, P, Hristova, M, Bennett, K, Lebon, S, Fleiss, B, Yellon, D, Hausenloy, D J, Golay, X & Robertson, N J 2015, ' Immediate remote ischemic postconditioning after hypoxia ischemia in piglets protects cerebral white matter but not grey matter ', Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism . https://doi.org/10.1177/0271678X15608862
ISSN: 1559-7016
0271-678X
DOI: 10.1177/0271678X15608862
Popis: Remote ischemic postconditioning (RIPostC) is a promising therapeutic intervention whereby brief episodes of ischemia/reperfusion of one organ (limb) mitigate damage in another organ (brain) that has experienced severe hypoxia-ischemia. Our aim was to assess whether RIPostC is protective following cerebral hypoxia-ischemia in a piglet model of neonatal encephalopathy (NE) using magnetic resonance spectroscopy (MRS) biomarkers and immunohistochemistry. After hypoxia-ischemia (HI), 16 Large White female newborn piglets were randomized to: (i) no intervention ( n = 8); (ii) RIPostC – with four, 10-min cycles of bilateral lower limb ischemia/reperfusion immediately after HI ( n = 8). RIPostC reduced the hypoxic-ischemic-induced increase in white matter proton MRS lactate/N acetyl aspartate ( p = 0.005) and increased whole brain phosphorus-31 MRS ATP ( p = 0.039) over the 48 h after HI. Cell death was reduced with RIPostC in the periventricular white matter ( p = 0.03), internal capsule ( p = 0.002) and corpus callosum ( p = 0.021); there was reduced microglial activation in corpus callosum ( p = 0.001) and more surviving oligodendrocytes in corpus callosum ( p = 0.029) and periventricular white matter ( p = 0.001). Changes in gene expression were detected in the white matter at 48 h, including KATP channel and endothelin A receptor. Immediate RIPostC is a potentially safe and promising brain protective therapy for babies with NE with protection in white but not grey matter.
Databáze: OpenAIRE