Immediate remote ischemic postconditioning after hypoxia ischemia in piglets protects cerebral white matter but not grey matter
Autor: | Xavier Golay, Aaron Oliver-Taylor, I Fierens, M Ezzati, Pierre Gressens, Daniel Alonso-Alconada, Kate Bennett, Nicola J. Robertson, Derek M. Yellon, Alan Bainbridge, Ilias Tachtsidis, Bobbi Fleiss, Kevin D. Broad, Jamshid Rostami, Sophie Lebon, K Jane Hassell, Mariya Hristova, Derek J. Hausenloy, Eridan Rocha-Ferreira, G Kawano |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Internal capsule Magnetic Resonance Spectroscopy Swine Gene Expression hypoxia–ischemia Corpus callosum remote ischemic postconditioning 0302 clinical medicine Adenosine Triphosphate KATP Channels Medicine Gray Matter Ischemic Postconditioning Brain Mapping neonatal encephalopathy Electroencephalography Receptor Endothelin A Immunohistochemistry White Matter 3. Good health medicine.anatomical_structure Neurology Lower Extremity Anesthesia Hypoxia-Ischemia Brain Cardiology neuroprotection Cardiology and Cardiovascular Medicine medicine.medical_specialty Ischemia Grey matter Neuroprotection White matter 03 medical and health sciences Internal medicine Animals Lactic Acid Aspartic Acid business.industry Neonatal encephalopathy Birth asphyxia Original Articles medicine.disease Disease Models Animal 030104 developmental biology Animals Newborn Neurology (clinical) business 030217 neurology & neurosurgery Biomarkers |
Zdroj: | Journal of Cerebral Blood Flow & Metabolism Ezzati, M, Bainbridge, A, Broad, K D, Kawano, G, Oliver-Taylor, A, Rocha-Ferreira, E, Alonso-Alconada, D, Fierens, I, Rostami, J, Jane Hassell, K, Tachtsidis, I, Gressens, P, Hristova, M, Bennett, K, Lebon, S, Fleiss, B, Yellon, D, Hausenloy, D J, Golay, X & Robertson, N J 2015, ' Immediate remote ischemic postconditioning after hypoxia ischemia in piglets protects cerebral white matter but not grey matter ', Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism . https://doi.org/10.1177/0271678X15608862 |
ISSN: | 1559-7016 0271-678X |
DOI: | 10.1177/0271678X15608862 |
Popis: | Remote ischemic postconditioning (RIPostC) is a promising therapeutic intervention whereby brief episodes of ischemia/reperfusion of one organ (limb) mitigate damage in another organ (brain) that has experienced severe hypoxia-ischemia. Our aim was to assess whether RIPostC is protective following cerebral hypoxia-ischemia in a piglet model of neonatal encephalopathy (NE) using magnetic resonance spectroscopy (MRS) biomarkers and immunohistochemistry. After hypoxia-ischemia (HI), 16 Large White female newborn piglets were randomized to: (i) no intervention ( n = 8); (ii) RIPostC – with four, 10-min cycles of bilateral lower limb ischemia/reperfusion immediately after HI ( n = 8). RIPostC reduced the hypoxic-ischemic-induced increase in white matter proton MRS lactate/N acetyl aspartate ( p = 0.005) and increased whole brain phosphorus-31 MRS ATP ( p = 0.039) over the 48 h after HI. Cell death was reduced with RIPostC in the periventricular white matter ( p = 0.03), internal capsule ( p = 0.002) and corpus callosum ( p = 0.021); there was reduced microglial activation in corpus callosum ( p = 0.001) and more surviving oligodendrocytes in corpus callosum ( p = 0.029) and periventricular white matter ( p = 0.001). Changes in gene expression were detected in the white matter at 48 h, including KATP channel and endothelin A receptor. Immediate RIPostC is a potentially safe and promising brain protective therapy for babies with NE with protection in white but not grey matter. |
Databáze: | OpenAIRE |
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