Intestinal changes associated with fluoride exposure in rats: Integrative morphological, proteomic and microbiome analyses
| Autor: | Carina Guimarães de Souza Melo, Tamara Teodoro Araujo, Talita Mendes da Silva Ventura, Isabela Tomazini Sabino-Arias, Jacqueline Nelisis Zanoni, Pamela K. Den Besten, Juliana Vanessa Colombo Martins Perles, Aline Dionizio, Marília Afonso Rabelo Buzalaf, Dawud Abduweli Uyghurturk |
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| Rok vydání: | 2021 |
| Předmět: |
Gene isoform
Proteomics Male Crohn’s disease medicine.medical_specialty Environmental Engineering Proteome Health Toxicology and Mutagenesis 0208 environmental biotechnology Firmicutes Ileum Inflammation 02 engineering and technology 010501 environmental sciences Biology 01 natural sciences Article Gastrointestinal symptoms Mice Fluorides Internal medicine Myosin medicine Environmental Chemistry Animals Meteorology & Atmospheric Sciences Microbiome Fluoride 0105 earth and related environmental sciences Crohn's disease Public Health Environmental and Occupational Health General Medicine General Chemistry medicine.disease Pollution 020801 environmental engineering Rats Gastrointestinal Microbiome Endocrinology medicine.anatomical_structure Toxicity Tunica medicine.symptom Environmental Sciences |
| Zdroj: | Chemosphere |
| Popis: | Gastrointestinal signs and symptoms are the first signs of toxicity due to exposure to fluoride (F). This suggests the possibility that lower levels of subchronic F exposure may affect the gut. The aim of this study was to evaluate changes in the morphology, proteome and microbiome of the ileum of rats, after subchronic exposure to F. Male rats ingested water with 0, 10, or 50mgF/L for thirty days. Treatment with F, regardless of the dose, significantly decreased the density of HuC/D-IR neurons, whereas CGRP-IR and SP-IR varicosities were significantly increased compared to the control group. Increased VIP-IR varicosities were significantly increased only in the group treated with 50mgF/L. A significant increase in thickness of the tunica muscularis, as well as in the total thickness of the ileum wall was observed at both F doses when compared to controls. In proteomics analysis, myosin isoforms were increased, and Gastrotopin was decreased in F-exposed mice. In the microbiome metagenomics analysis, Class Clostridia was significantly reduced upon exposure to 10mgF/L. At the higher F dose of 50mg/L, genus Ureaplasma was significantly reduced in comparison with controls. Morphological and proteomics alterations induced by F were marked by changes associated with inflammation, and alterations in the gut microbiome. Further studies are needed to determine whether F exposure increases inflammation with secondary effects of the gut microbiome, and/or whether primary effects of F on the gut microbiome enhance changes associated with inflammation. |
| Databáze: | OpenAIRE |
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