Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato
| Autor: | Johann Petit, Lucie Fernandez, Joana Jorly, Yoshihiro Okabe, Pierre Baldet, Hiroshi Ezura, Martine Lemaire-Chamley, Julien Assali, Kentaro Mori, Virginie Garcia, Cécile Bres, Christophe Rothan, Jean-Philippe Mauxion, Carine Ferrand, Céline Bournonville, Tohru Ariizumi, Daniel Just, Erika Asamizu |
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| Přispěvatelé: | Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Université Sciences et Technologies - Bordeaux 1-Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA), Université de Tsukuba = University of Tsukuba |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
TILLING provide insight Candidate gene Ethyl methanesulfonate phenotype [SDV]Life Sciences [q-bio] Mutant Plant Science Biology tomato 01 natural sciences Genome Deep sequencing cultivated tomato 03 medical and health sciences chemistry.chemical_compound deep sequencing tilling cuticle formation [SDV.BV]Life Sciences [q-bio]/Vegetal Biology mutant database ComputingMilieux_MISCELLANEOUS 030304 developmental biology 2. Zero hunger Genetics 0303 health sciences Genetic diversity fungi reverse genetic food and beverages candidate gene Forward genetics arabidopsis chemistry fruit development point mutation induced mutation forward genetic Agronomy and Crop Science model system 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Biotechnology Plant Biotechnology, Japanese Society for Plant Cell and Molecular Biology, 2013, 30 (3), pp.225-231. ⟨10.5511/plantbiotechnology.13.0622a⟩ |
| ISSN: | 1342-4580 1347-6114 |
| DOI: | 10.5511/plantbiotechnology.13.0622a⟩ |
| Popis: | International audience; Tomato is currently the model plant for fleshy fruit development and for Solanaceae species. Recent genomic approaches including transcriptome, proteome and metabolome analyses and genetic mapping have produced a wealth of candidate genes whose function needs to be assessed. The recent development in model and crop plants of TILLING (Targeting Induced Local Lesions IN Genomes), which reveals allelic series corresponding to several independent point mutations, and the current availability of deep sequencing tools further increase the interest of generating artificially-induced genetic diversity in tomato. We describe here the generation and use of EMS (ethyl methanesulfonate) tomato mutants in the miniature cultivar Micro-Tom and provide as example the identification of new fruit size and morphology mutants. We further propose new deep sequencing-based strategies for the discovery of mutations underlying phenotypic variations observed in mutant collections that will considerably increase the interest of exploiting Micro-Tom mutant collections for gene discovery in tomato. |
| Databáze: | OpenAIRE |
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