FAS is highly expressed in the germinal center but is not required for regulation of the B-cell response to antigen
| Autor: | G. J. V. Nossal, David M. Tarlinton, Kenneth G. C. Smith |
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| Rok vydání: | 1995 |
| Předmět: |
Molecular Sequence Data
Antibody Affinity Immunoglobulin Variable Region Biology Fas ligand Nitrophenols Mice Immune system Antigen Sequence Homology Nucleic Acid medicine Animals Amino Acid Sequence fas Receptor Selection Genetic Antibody-Producing Cells B cell Phenylacetates B-Lymphocytes Multidisciplinary Base Sequence Sequence Homology Amino Acid Germinal center Germinal Center Fas receptor Molecular biology Mice Mutant Strains Mice Inbred C57BL medicine.anatomical_structure Apoptosis Antibody Formation Mutation biology.protein Antibody Immunoglobulin Heavy Chains Haptens Immunologic Memory Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 92:11628-11632 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.92.25.11628 |
| Popis: | In establishing the memory B-cell population and maintaining self-tolerance during an immune response, apoptosis mediates the removal of early, low-affinity antibody-forming cells, unselected germinal center (GC) cells, and, potentially, self-reactive B cells. To address the role of the apoptosis-signaling cell surface molecule FAS in the B-cell response to antigen, we have examined the T-cell-dependent B-cell response to the carrier-conjugated hapten (4-hydroxy-3-nitrophenyl)acetyl (NP) in lpr mice in which the fas gene is mutated. High levels of FAS were expressed on normal GC B cells but the absence of FAS did not perturb the progressive decline in numbers of either GC B cells or extrafollicular antibody-forming cells. Furthermore, the rate of formation and eventual size of the NP-specific memory B-cell population in lpr mice were normal. The accumulation of cells with affinity-enhancing mutations and the appearance of high-affinity anti-NP IgG1 antibody in the serum were also normal in lpr mice. Thus, although high levels of FAS are expressed on GC B cells, FAS is not required for GC selection or for regulation of the major antigen-specific B-cell compartments. The results suggest that the size and composition of B-cell compartments in the humoral immune response are regulated by mechanisms that do not require FAS. |
| Databáze: | OpenAIRE |
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