Environmental enrichment attenuates the age-related decline in the mRNA expression of steroidogenic enzymes and reduces the methylation state of the steroid 5α-reductase type 1 gene in the rat hippocampus
| Autor: | Guillermo S. Moreno-Piovano, Jorgelina Varayoud, María Florencia Rossetti, Jorge G. Ramos, Enrique H. Luque |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Aging CIENCIAS MÉDICAS Y DE LA SALUD Neurociencias Gene Expression Biology CREB Dna Methylation Biochemistry Hippocampus Epigenesis Genetic Neurosteroidogenic Enzymes Endocrinology 3-Oxo-5-alpha-Steroid 4-Dehydrogenase Internal medicine Gene expression Environmental Enrichment medicine Animals Epigenetics RNA Messenger Rats Wistar Promoter Regions Genetic Molecular Biology Environmental enrichment Cholesterol side-chain cleavage enzyme Cytochrome P450 Methylation DNA Methylation 3-alpha-Hydroxysteroid Dehydrogenase (B-Specific) Housing Animal Biosynthetic Pathways Medicina Básica DNA methylation biology.protein Female |
| Zdroj: | Molecular and cellular endocrinology. 412 |
| ISSN: | 1872-8057 |
| Popis: | We analyzed the effects of aging and environmental enrichment on the mRNA expression and DNA methylation state of steroidogenic enzymes in the hippocampus. The effects of agingwere evaluated by comparing young adult (90-day-old) and middle-aged (450-day-old) female Wistar rats. To elucidate the effects of environmental enrichment, a subgroup of middle-aged rats exposed to sensory and social stimulation for 105 days was compared to rats housed under standard laboratory conditions. Aging decreased the transcription of neurosteroidogenic-related genes and increased the promoter methylation state of cytochrome P450 side chain cleavage, 3α-hydroxysteroid dehydrogenase (3α-HSD) and 5α-reductase-1. Exposure of middle-aged rats to environmental enrichment increased mRNA levels of 5α-reductase-1, 3α-HSD and cytochrome P450 17α-hydroxylase/c17,20-lyase and decreased the methylation state of the 5α-reductase-1 gene. Thus, sensory and social stimulation attenuate the age-related decline in the mRNA expression of hippocampal steroidogenic enzymes. Epigenetic mechanisms associated with differential promoter methylation could be involved. Fil: Rossetti, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Varayoud, Jorgelina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Moreno Piovano, Guillermo Samuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Luque, Enrique Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina Fil: Ramos, Jorge Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; Argentina |
| Databáze: | OpenAIRE |
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