Growth hormone potentiates thyroid hormone effects on post-exercise phosphocreatine recovery in skeletal muscle

Autor: E. Jeannesson, Pierre Kaminsky, F Barbé, Joelle Deibener, Marc Klein, Jean Marie Escanyé, Paul Walker, Brigitte Dousset
Přispěvatelé: Service de médecine interne, Centre Hospitalier Régional Universitaire de Nancy ( CHRU Nancy ), Laboratoire Electronique, Informatique et Image ( Le2i ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Pôle des Spécialités Médicales, Laboratoire de Biochimie des Protéines, Service de Médecine Nucléaire [Nancy], laboratoire de methodologie RMN, Université Henri Poincaré - Nancy 1 ( UHP ) -Centre National de la Recherche Scientifique ( CNRS ), Service de Biochimie et Biologie Moléculaire, Nutrition et Métabolisme [CHRU Nancy], Service d'Endocrinologie [CHRU Nancy], Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Laboratoire Electronique, Informatique et Image [UMR6306] (Le2i), Université de Bourgogne (UB)-École Nationale Supérieure d'Arts et Métiers (ENSAM), Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-Arts et Métiers Sciences et Technologies, HESAM Université (HESAM)-HESAM Université (HESAM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Université Henri Poincaré - Nancy 1 (UHP)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2011
Předmět:
Male
Phosphocreatine
Thyroid hormones
Endocrinology
Diabetes and Metabolism
MESH: Random Allocation
Thyroid Gland
Skeletal muscle
Hypopituitarism
MESH: Physical Conditioning
Animal
MESH: Drug Synergism
Nuclear magnetic resonance
chemistry.chemical_compound
Random Allocation
0302 clinical medicine
Endocrinology
MESH: Human Growth Hormone
MESH: Animals
MESH : Muscle
Skeletal
0303 health sciences
MESH: Muscle
Skeletal
[ INFO.INFO-IM ] Computer Science [cs]/Medical Imaging
MESH : Rats
Human Growth Hormone
Thyroid
Drug Synergism
medicine.anatomical_structure
medicine.drug
medicine.medical_specialty
MESH : Drug Synergism
MESH: Rats
MESH : Male
Somatotropin
030209 endocrinology & metabolism
MESH: Phosphocreatine
MESH : Random Allocation
03 medical and health sciences
In vivo
Internal medicine
[ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology
Physical Conditioning
Animal
MESH : Thyroxine
medicine
[INFO.INFO-IM]Computer Science [cs]/Medical Imaging
Animals
Humans
MESH : Phosphocreatine
MESH : Human Growth Hormone
Mitochondrion
MESH : Physical Conditioning
Animal
Muscle
Skeletal
030304 developmental biology
Hydrocortisone
MESH: Humans
MESH : Humans
MESH : Thyroid Gland
MESH: Thyroxine
medicine.disease
MESH: Male
MESH: Thyroid Gland
Rats
Thyroxine
chemistry
Rat
MESH : Animals
Tetanic stimulation
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Hormone
Zdroj: Growth Hormone & IGF Research
Growth Hormone & IGF Research, 2012, 22 (6), pp.240-244. 〈10.1016/j.ghir.2012.08.001〉
Growth Horm IGF Res
Growth Horm IGF Res, 2012, 22 (6), pp.240-4. ⟨10.1016/j.ghir.2012.08.001⟩
Growth Hormone & IGF Research, 2012, 22 (6), pp.240-244. ⟨10.1016/j.ghir.2012.08.001⟩
Growth Horm IGF Res, 2012, 22 (6), pp.240-4. 〈10.1016/j.ghir.2012.08.001〉
ISSN: 1532-2238
DOI: 10.1016/j.ghir.2012.08.001〉
Popis: International audience; OBJECTIVE: The aim of the study was to determine the respective impact of thyroxine and growth hormone on in vivo skeletal mitochondrial function assessed via post exercise phosphocreatine recovery. DESIGN: The hind leg muscles of 32 hypophysectomized rats were investigated using (31)P nuclear magnetic resonance spectroscopy at rest and during the recovery period following a non tetanic stimulation of the sciatic nerve. Each rat was supplemented with hydrocortisone and was randomly assigned to one of the 4 groups: the group Hx was maintained in hypopituitarism., the group HxT was treated with 1 μg/100g/day of thyroxine (T4), the group HxG with 0.2 IU/kg/day of recombinant human GH (rGH) and the group HxGT by both thyroxine and rGH. Inorganic phosphate (Pi), phosphocreatine (PCr) and ATP were directly measured on the spectra, permitting the calculation of the phosphorylation potential (PP). RESULTS: At rest, the rats treated with rGH or T4 exhibited higher PCr levels than rats Hx. The recovery rates of PCr and PP were higher in rats treated with T4 than in T4-deprivated rats, suggesting improved mitochondrial function. The rats treated by both T4 and rGH showed higher PCr and PP recovery than those maintained in hypopituitarism or treated with T4 or rGH alone. CONCLUSIONS: The study demonstrates that in contrast to T4, GH given alone in hypophysectomized rats does not improve in vivo mitochondrial oxidative metabolism. Growth hormone potentiates T4 effects on oxidative metabolism.
Databáze: OpenAIRE
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