Keratocyte injury in human corneas cryopreserved under standard conditions
| Autor: | Rafael Villalba, J.L. Gómez-Villagrán, J. Peña, J.M. Villalba, Evelio Luque |
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| Rok vydání: | 2004 |
| Předmět: |
Cryopreservation
Transplantation medicine.medical_specialty Pathology Necrosis Biomedical Engineering Cellular death Cell Biology Biology Surgery Biomaterials Cornea medicine.anatomical_structure Apoptosis medicine Necrotic Process Standard protocol Microscopy Electron Scanning Humans sense organs medicine.symptom |
| Zdroj: | Cell and tissue banking. 5(4) |
| ISSN: | 1389-9333 |
| Popis: | This study was conducted to characterize ultrastructural damage to human corneas cryopreserved by a standard protocol. The materials used were seven human corneas that were unsuitable for transplantation due to the presence of positive bacteriological cultures; they were cryopreserved according the standard procedure. After freezing and thawing, samples were obtained for scanning and transmission electron microscopy studies. Marked damage was observed in keratocytes with signs of apoptotic cellular injury. However our observations have shown that apoptosis contribute less significantly than necrosis to cellular death in keratocytes of human corneas and although the control of apoptosis is clearly desirable, in order to improve the success of cryopreserved corneas for transplant, we need to continue our investigation to reduce the effects of the necrotic process. |
| Databáze: | OpenAIRE |
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