Follicle stimulating hormone (FSH) and luteinizing hormone (LH) gene expression during larval development in Senegalese sole (Solea senegalensis)
| Autor: | Jesús Ramos, Carmen Sarasquete, José M. Guzmán, Yonathan Zohar, María J. Bayarri, Evaristo L. Mañanós |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
LH
endocrine system medicine.medical_specialty Physiology medicine.drug_class Ontogeny media_common.quotation_subject Solea senegalensis Early development Biology Biochemistry Follicle-stimulating hormone Larvae Internal medicine Gene expression FSH medicine Animals Metamorphosis Molecular Biology media_common Regulation of gene expression Gonadotropin Hatching Metamorphosis Biological Gene Expression Regulation Developmental Luteinizing Hormone beta Subunit Luteinizing Hormone Endocrinology Glycoprotein Hormones alpha Subunit Larva Flatfishes Female Follicle Stimulating Hormone Luteinizing hormone Senegalese sole hormones hormone substitutes and hormone antagonists |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 7 páginas, 4 figuras, 1 tabla. The gonadotropins (GTHs), follicle stimulating hormone (FSH) and luteinizing hormone (LH), determine the reproductive competence of adult breeders, but also participate in the establishment of the reproductive axis at early stages of life. The present study aimed at studying, by real-time qPCR, the gene expression levels of GTH subunits (FSHβ, LHβ and the common glycoprotein α -GPα- subunit) during early development in Senegalese sole, from 1 to 100 days post hatching (dph). The FSHβ, LHβ and GPα transcripts were first detected at 1, 5 and 3 dph, respectively. Transcript levels of FSHβ, and GPα, increased continuously to peak levels at mid metamorphosis (15 dph), decreasing thereafter; levels were maintained low until a second increment detected at 90 and 100 dph. Contrarily, transcript levels of LHβ were very low and only detectable around metamorphosis. All three subunits were highly expressed in 1-year old soles, with FSHβ and GPα transcript levels 10-fold higher than those of LHβ. These results suggest, i) activity of the reproductive axis early after hatching (1 dph), which was highest during the metamorphic climax and, ii) a predominant role of FSH, rather than LH, in the early development of the reproductive axis in Senegalese sole. This research was funded by the Spanish Ministry of Education and Science (MEC) (proj. AGL2006-13777) and the Ministry of Agriculture, Fisheries and Food (MAPA) (proj. JACUMAR 2006, II National plan for the culture of sole). J.M. Guzmán received a FPI fellowship from the MEC. M.J. Bayarri enjoyed a postdoctoral research grant by L'Oreal-UNESCO “For Women in Science”. |
| Databáze: | OpenAIRE |
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