DIDS inhibits overexpression BAK1-induced mitochondrial apoptosis through GSK3β/β-catenin signaling pathway
| Autor: | Xiayun Yang, Xiaohong Yu, Shusheng Tang, Daowen Li, Fuyun Wang, Xilong Xiao |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Poly ADP ribose polymerase Clinical Biochemistry Apoptosis 4 4'-Diisothiocyanostilbene-2 2'-Disulfonic Acid 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation Humans Phosphorylation Wnt Signaling Pathway Glycogen Synthase Kinase 3 beta Chemistry Activator (genetics) fungi Wnt signaling pathway Cell Biology Cell biology Mitochondria Up-Regulation 030104 developmental biology HEK293 Cells bcl-2 Homologous Antagonist-Killer Protein DIDS Chloride channel Signal transduction |
| Zdroj: | Journal of cellular physiology. 233(6) |
| ISSN: | 1097-4652 |
| Popis: | Bcl-2 homologous antagonist/killer (BAK1) is a critical regulator of mitochondrial apoptosis. Although upregulation of BAK1 induces apoptosis has been established, the underlying molecular mechanism is far from clear. 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), an organic anion used as a blocker of anion exchangers and chloride channels, has been proved to rescue cell apoptosis both in vitro and in vivo. However, whether DIDS can inhibit BAK1-induced mitochondrial apoptosis remains undefined. Thus, this study aimed to explore whether DIDS could protect BAK1-induced apoptosis through GSK3β/β-catenin signaling pathway. The results showed overexpression BAK1 in 293T cells induced mitochondrial apoptosis accompanied by increasing the expression levels of cleaved caspase-9, -3, poly (ADP-ribose) polymerase (PARP) and reducing the MMP. Furthermore, overexpression BAK1 decreased the expression levels of Ser9-GSK3β and β-catenin. In addition, lithium chloride (LiCl), an activator of Wnt/β-catenin signaling pathway, markedly attenuated overexpression BAK1-induced mitochondrial apoptosis by restoring the expression levels of Ser9-GSK3β and β-catenin. Finally, DIDS absolutely abolished overexpression BAK1-mediated mitochondrial apoptosis through recovering the expression levels of Ser9-GSK3β and β-catenin. Taken together, our results reveal that DIDS blocks overexpression BAK1-induced mitochondrial apoptosis through GSK3β/β-catenin pathway. |
| Databáze: | OpenAIRE |
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