Evaluation of Stability and In Vitro Security of Nanoemulsions Containing Eucalyptus globulus Oil
| Autor: | Aline Ferreira Ourique, Priscilla Maciel Quatrin, Bruna Klein, Roger Wagner, Roberto Christ Vianna Santos, Kátia Nascimento, Samantha Nunes de Godoi, Michele Rorato Sagrillo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Article Subject Stability study lcsh:Medicine 02 engineering and technology General Biochemistry Genetics and Molecular Biology law.invention 03 medical and health sciences law Food science Cell survival Essential oil Volume concentration General Immunology and Microbiology biology business.industry Chemistry lcsh:R General Medicine 021001 nanoscience & nanotechnology biology.organism_classification In vitro Biotechnology 030104 developmental biology Eucalyptus globulus Free form 0210 nano-technology business |
| Zdroj: | BioMed Research International, Vol 2017 (2017) |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2017/2723418 |
| Popis: | Essential oil of Eucalyptus globulus presents several pharmacological properties. However, their therapeutic efficacy may be affected by limitations due to several conditions, rendering it difficult to obtain stable and effective pharmaceutical formulations. The use of nanotechnology is an alternative to improve their characteristics aiming to ensure their stability and effectiveness. Furthermore, studies about the possible toxic effects of nanostructures are necessary to evaluate safety when the formulation comes into contact with human cells. Hence, in this paper, we evaluate for the first time the stability and in vitro cytogenotoxicity of nanoemulsions containing Eucalyptus globulus in peripheral blood mononuclear cells. As a result, the stability study found that the best condition for storage up to 90 days was refrigeration (4°C); it was the condition that best preserved the nanometric features. The content of the major compounds of oil was maintained after nanoencapsulation and preserved over time. In tests to evaluate the safety of this formulation, we can conclude that, at a low concentration (approximately 0.1%), Eucalyptus globulus nanoemulsion did not cause toxicity in peripheral blood mononuclear cells and also showed a protective effect in cells against possible damage when compared to oil in free form. |
| Databáze: | OpenAIRE |
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