Lignin degradation and detoxification of eucalyptus wastes by on-site manufacturing fungal enzymes to enhance second-generation ethanol yield
| Autor: | Alicia Prieto, Ana Gutiérrez, Willian Daniel Hahn Schneider, Félix Gonçalves de Siqueira, María Jesús Martínez, Henrique Macedo Baudel, Laura I. de Eugenio, Aldo José Pinheiro Dillon, Marli Camassola, Roselei Claudete Fontana, Ángel T. Martínez, Jorge Rencoret |
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| Přispěvatelé: | Agencia Estatal de Investigación (España), Universidade de Caxias do Sul, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Comunidad de Madrid, Empresa Brasileira de Pesquisa Agropecuária, Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Consejo Superior de Investigaciones Científicas (España), de Siqueira, Félix Gonçalves [0000-0001-5239-4994], Rencoret, Jorge [0000-0003-2728-7331], Gutiérrez Suárez, Ana [0000-0002-8823-9029], Martínez, Ángel T. [0000-0002-1584-2863], de Siqueira, Félix Gonçalves, Rencoret, Jorge, Gutiérrez Suárez, Ana, Martínez, Ángel T. |
| Rok vydání: | 2020 |
| Předmět: |
020209 energy
Bioethanol 02 engineering and technology Management Monitoring Policy and Law Raw material Saccharification complex mixtures chemistry.chemical_compound Hydrolysis 020401 chemical engineering Enzymatic hydrolysis 0202 electrical engineering electronic engineering information engineering Lignin 0204 chemical engineering Secretome Laccase biology Mechanical Engineering technology industry and agriculture food and beverages Marasmiellus palmivorus Building and Construction biology.organism_classification Pulp and paper industry General Energy chemistry Biofuel Eucalyptus globulus Fermentation White-rot basidiomycete |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 12 páginas.- 5 figuras.- 4 tablas.- 103 referencias.- Supplementary data to this article can be found online at https://doi.org/10.1016/j.apenergy.2020.114493.0.00.51.01.52.0 Novel laccases have promising and valuable applications in biorefineries. This investigation documents, for the first time, the potential of depolymerising and repolymerising lignin by the secretome, rich in laccases, from a newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111, for further saccharification and ethanolic fermentation steps. Proteomic analyses of the secretome of M. palmivorus show that laccases are the most predominant enzyme released by this fungus. The whole crude enzymatic broth is used for the delignification of lignin in Eucalyptus globulus wood, with the aim of enhancing the saccharification by cellulolytic and xylanolytic enzymes from Penicillium echinulatum S1M29. In addition, two different strategies, namely, laccase treatment before and after enzymatic hydrolysis, are employed to detoxify steam-exploded E. globulus wood. The objective is to increase the fermentative performance by removing substances formed during the feedstock pretreatment that can inhibit microbial fermentation. The E. globulus wood delignification results in a 31% decrease in the lignin content and a 10% increase in the glucose yield after hydrolysis. An important finding of the present work is the successful wood delignification in the absence of laccase mediators. This laccase-rich preparation also demonstrates its potential in removing the phenolic inhibitors present in steam-exploded E. globulus wood, increasing the ethanol yield by an additional 10%. Furthermore, it is important to highlight that these findings are achieved in the absence of commercial enzymes, making M. palmivorus laccases a potential candidate not only for the production of biofuels but also for the generation of lignin-derived aromatic compounds for different applications in the biotechnology industry. © 2020 Elsevier Ltd Funding from University of Caxias do Sul (UCS) Coordination of Improvement of Higher Education Personnel (CAPES) (Process 3255/2013), Research Support Foundation of the State of Rio Grande do Sul (Process 16/2551-0000 246-0), Brazilian Agricultural Research Corporation (EMBRAPA) (34/2014), National Council for Scientific and Technological Development (CNPq) (Process 201925/2018-4), the Biobased Industries Joint Undertaking (www.bbi-europe.eu) project EnzOx2 (H2020-BBI-PPP-2015-2-720297, www.enzox2.eu), the projects RETOPROSOST-2-CM [S2018/EMT-4459] financed by the Comunidad de Madrid, HIDROFUN [BIO2015-68387-R], and GLYSUS [RTI2018-093683-B-I00] from MINECO/FEDER, and the CSIC Project (201740E07) |
| Databáze: | OpenAIRE |
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