Microbiota-Derived Extracellular Vesicles Detected in Human Blood from Healthy Donors
| Autor: | Béatrice Schaack, Thomas Hindré, Nyamekye Quansah, Dalil Hannani, Corinne Mercier, David Laurin |
|---|---|
| Přispěvatelé: | Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 (TIMC ), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), ANR-15-IDEX-0002,UGA,IDEX UGA(2015) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Erythrocytes
Health Status membrane fusion MESH: Monocytes red blood cell concentrates Catalysis Monocytes Inorganic Chemistry Extracellular Vesicles Escherichia coli Humans MESH: Microbiota Physical and Theoretical Chemistry MESH: Extracellular Vesicles Molecular Biology Spectroscopy MESH: Health Status MESH: Humans gut microbiota outer membrane vesicles (OMVs) [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry Molecular Biology/Structural Biology [q-bio.BM] MESH: Escherichia coli Microbiota MESH: Erythrocytes Organic Chemistry General Medicine Computer Science Applications OmpA extracellular vesicles (EVs) lipopolysaccharide (LPS) |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2022, 23 (22), pp.13787. ⟨10.3390/ijms232213787⟩ International Journal of Molecular Sciences; Volume 23; Issue 22; Pages: 13787 |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms232213787⟩ |
| Popis: | International audience; The microbiota constitutes an important part of the holobiont in which extracellular vesicles (EVs) are key players in health, especially regarding inter- and intra-kingdom communications. Analysis of EVs from the red blood cell concentrates of healthy donors revealed variable amounts of OmpA and LPS in 12 of the 14 analyzed samples, providing indirect experimental evidence of the presence of microbiota EVs in human circulating blood in the absence of barrier disruption. To investigate the role of these microbiota EVs, we tracked the fusion of fluorescent Escherichia coli EVs with blood mononuclear cells and showed that, in the circulating blood, these EVs interacted almost exclusively with monocytes. This study demonstrates that bacterial EVs constitute critical elements of the host–microbiota cellular communication. The analysis of bacterial EVs should thus be systematically included in any characterization of human EVs. |
| Databáze: | OpenAIRE |
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