Identification and Characterization of microRNAs during Retinoic Acid-Induced Regeneration of a Molluscan Central Nervous System
| Autor: | Robert L. Carlone, Sarah E. Walker, Gaynor E. Spencer, Aleksandar Necakov |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Nervous system Central Nervous System Neurite Central nervous system Growth Cones Retinoic acid Tretinoin Biology Catalysis Article lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation microRNA Lymnaea medicine retinoic acid Animals Physical and Theoretical Chemistry Growth cone lcsh:QH301-705.5 Molecular Biology Spectroscopy Neurons neuronal regeneration Regeneration (biology) Organic Chemistry RNA sequencing General Medicine Computer Science Applications Cell biology growth cone MicroRNAs 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) lcsh:QD1-999 chemistry Mollusca physiology |
| Zdroj: | International Journal of Molecular Sciences Volume 19 Issue 9 International Journal of Molecular Sciences, Vol 19, Iss 9, p 2741 (2018) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms19092741 |
| Popis: | Retinoic acid (RA) is the biologically active metabolite of vitamin A and has become a well-established factor that induces neurite outgrowth and regeneration in both vertebrates and invertebrates. However, the underlying regulatory mechanisms that may mediate RA-induced neurite sprouting remain unclear. In the past decade, microRNAs have emerged as important regulators of nervous system development and regeneration, and have been shown to contribute to processes such as neurite sprouting. However, few studies have demonstrated the role of miRNAs in RA-induced neurite sprouting. By miRNA sequencing analysis, we identify 482 miRNAs in the regenerating central nervous system (CNS) of the mollusc Lymnaea stagnalis, 219 of which represent potentially novel miRNAs. Of the remaining conserved miRNAs, 38 show a statistically significant up- or downregulation in regenerating CNS as a result of RA treatment. We further characterized the expression of one neuronally-enriched miRNA upregulated by RA, miR-124. We demonstrate, for the first time, that miR-124 is expressed within the cell bodies and neurites of regenerating motorneurons. Moreover, we identify miR-124 expression within the growth cones of cultured ciliary motorneurons (pedal A), whereas expression in the growth cones of another class of respiratory motorneurons (right parietal A) was absent in vitro. These findings support our hypothesis that miRNAs are important regulators of retinoic acid-induced neuronal outgrowth and regeneration in regeneration-competent species. |
| Databáze: | OpenAIRE |
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