Expression of thyroid hormone receptor isoforms in rat oligodendrocyte cultures. Effect of 3,5,3'-triiodo-L-thyronine
| Autor: | Jack Puymirat, C. Fressinaud, D. Dalençon, Marie Elisabeth Ittel, A. Reeber, Sarlieve Ll, Dominique Baas |
|---|---|
| Přispěvatelé: | Deleage, Gilbert |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Fluorescent Antibody Technique Gene Expression Biology chemistry.chemical_compound Internal medicine Gene expression medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals RNA Messenger Receptor Cells Cultured Cell Nucleus Messenger RNA Triiodothyronine Thyroid hormone receptor Receptors Thyroid Hormone General Neuroscience Lineage markers Brain Blotting Northern Oligodendrocyte Rats Oligodendroglia medicine.anatomical_structure Endocrinology chemistry Animals Newborn Thyronine |
| Popis: | 3,5,3'-Triiodo-L-thyronine (T3) acts at the genomic level by interacting with nuclear T3 receptors (T3Rs). We have used double immunostaining to follow the expression of T3Rs and oligodendrocytes (OL) lineage markers in rat secondary cultures consisting of 85-90% OL. Using antibodies against different synthetic peptides of T3Rs (alpha common: alpha 1 + alpha 2 and beta 1) we find that alpha-T3R is expressed in both O-2A progenitors and in mature OL, while beta 1-T3R is expressed only in mature OL. In cultured OL, beta 1-T3R mRNA is upregulated the most by T3. OL exhibit more numerous and longer processes when treated by T3.3,5,3'-Triiodo-L-thyronine (T3) acts at the genomic level by interacting with nuclear T3 receptors (T3Rs). We have used double immunostaining to follow the expression of T3Rs and oligodendrocytes (OL) lineage markers in rat secondary cultures consisting of 85-90% OL. Using antibodies against different synthetic peptides of T3Rs (alpha common: alpha 1 + alpha 2 and beta 1) we find that alpha-T3R is expressed in both O-2A progenitors and in mature OL, while beta 1-T3R is expressed only in mature OL. In cultured OL, beta 1-T3R mRNA is upregulated the most by T3. OL exhibit more numerous and longer processes when treated by T3. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|