Anchorage-independent growth of Ewing sarcoma cells under serum-free conditions is not associated with stem-cell like phenotype and function
| Autor: | Jutta Meltzer, A. Luecke, Dagmar Clemens, Jenny Potratz, Bianca Altvater, Heribert Juergens, Claudia Rossig, Sareetha Kailayangiri, Simeon Hoffschlag, Uta Dirksen, K. Leuchte, Jendrik Hardes |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
CD99 Cell Cell Culture Techniques Sarcoma Ewing Biology Stem cell marker Culture Media Serum-Free Mice Mice Inbred NOD Cell Line Tumor Spheroids Cellular medicine Biomarkers Tumor Tumor Cells Cultured Animals Humans Neoplasm Metastasis Oncogene General Medicine Neoplasms Experimental Cell cycle medicine.disease medicine.anatomical_structure Oncology Cell culture Cancer research Neoplastic Stem Cells Sarcoma Stem cell |
| Zdroj: | Oncology reports. 32(2) |
| ISSN: | 1791-2431 |
| Popis: | Novel treatment strategies for Ewing sarcoma aim to eliminate residual tumor cells that have maintained the capacity to reinitiate tumor growth after intensive conventional therapy. Preclinical models that more closely mimic in vivo tumor growth than standard monolayer cultures are needed. Sphere formation under anchorage-independent, serum-free conditions has been proposed to enrich for cells with tumor-initiating, stem cell-like properties in various solid cancers. In the present study, we assessed the phenotype and functional stem cell characteristics of Ewing sarcoma spheres. Spheres were generated under serum-free culture conditions from four Ewing sarcoma cell lines and four relapse tumor biopsies. Standard monolayer cultures were established as controls. Median levels of surface expression of the Ewing sarcoma marker CD99 as well as the supposed stem cell marker CD133 and the neural crest marker CD57 were comparable between spheres and monolayers. Ewing sarcoma spheres from individual tumors failed to continuously self-renew by secondary sphere formation. They contained variable proportions of side populations (SPs). Sphere culture did not enhance the in vivo tumorigenicity of Ewing sarcoma cells in a murine xenograft model. We conclude that sphere formation under serum-free conditions is not a reliable tool to enrich for cells with stem cell characteristics in Ewing sarcoma. By mimicking the anchorage-independent, multicellular growth of Ewing sarcoma micrometastases, in vitro sphere growth may still add value as a preclinical tool to evaluate the efficacy of novel therapeutics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|