Emergent heterogeneity in putative mesenchymal stem cell colonies: Single-cell time lapsed analysis
| Autor: | Joana S Raminhos, Krystyn J. Van Vliet, Samantha M Leff, Pristinavae Manning, Deena A. Rennerfeldt |
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| Přispěvatelé: | DCM - Departamento de Ciência dos Materiais |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Intravital Microscopy medicine.medical_treatment Twins Cell therapy 0302 clinical medicine Mathematical and Statistical Techniques Single-cell analysis Animal Cells Cell Cycle and Cell Division Cells Cultured Cell Analysis Principal Component Analysis Multidisciplinary Agricultural and Biological Sciences(all) Stem Cells Physics Statistics Stem-cell therapy Cell biology medicine.anatomical_structure Bioassays and Physiological Analysis Cell Division Analysis Cell Processes Physical Sciences Medicine Stem cell Single-Cell Analysis Cellular Types Cell Division Research Article Stromal cell Imaging Techniques Science Biophysics Bone Marrow Cells Image Analysis Biology Research and Analysis Methods Time-Lapse Imaging Colony-Forming Units Assay 03 medical and health sciences medicine Humans Statistical Methods General Biochemistry Genetics and Molecular Biology(all) Mesenchymal stem cell Biology and Life Sciences Mesenchymal Stem Cells Cell Biology Single mothers 030104 developmental biology Multivariate Analysis Bone marrow 030217 neurology & neurosurgery Mathematics Developmental Biology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 14, Iss 4, p e0213452 (2019) Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1932-6203 |
| Popis: | Grant No. 1122374 Bone marrow stromal cells (BMSCs) include a subset of stem cells that are considered promising for developmental studies and therapeutic applications. While it is appreciated generally that BMSC populations can exhibit morphological and functional heterogeneity upon in vitro culture expansion, the potential for heterogeneity within a single colony forming unit–generated ostensibly from a single mother cell–is less explored but is critical to design of both fundamental studies and cell therapy production. Here we observed BMSC colony formation in real time via time lapsed optical imaging and analysis, to quantify whether and how heterogeneity emerged over multiple cell divisions spanning the duration of a typical colony formation unit assay. These analyses demonstrate that such colonies are neither homogeneous subpopulations of stem cells nor necessarily derived from single originating cells. While the mechanisms for and causes of this intracolony heterogeneity are not understood fully, we further demonstrate that extensive cell-cell contacts do not correlate with senescence, but that media exchange was concurrent with diversification in even the most uniform single-cell-derived colonies. These direct quantitative observations and visualizations of colony formation provide new insights that are motivated by significant implications for both basic research and stem cell-based therapies. publishersversion published |
| Databáze: | OpenAIRE |
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