MEK-ERK is involved in SUMO-1 foci formation on apoptosis
| Autor: | Kiyotsugu Kojima, Ryoko Utsubo-Kuniyoshi, Natsuhiko Sugimura, Akiko Rokudai, Kiyohiko Hatake, Yoshiko Sonoda, Tadashi Kasahara, Yuko Mishima, Yuji Mishima, Yasuhito Terui |
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| Rok vydání: | 2007 |
| Předmět: |
MAPK/ERK pathway
Cancer Research Programmed cell death Small interfering RNA SUMO-1 Protein genetic processes SUMO protein Apoptosis macromolecular substances Biology Transfection environment and public health Nitriles Butadienes Humans Enzyme Inhibitors RNA Small Interfering Extracellular Signal-Regulated MAP Kinases Adaptor Proteins Signal Transducing Mitogen-Activated Protein Kinase Kinases MEK inhibitor General Medicine Molecular biology enzymes and coenzymes (carbohydrates) Oncology Small ubiquitin-related modifier 1 embryonic structures Fatty Acids Unsaturated Tetradecanoylphorbol Acetate Mitogen-Activated Protein Kinases Mitoxantrone K562 Cells Signal Transduction |
| Zdroj: | Cancer Science. 98:569-576 |
| ISSN: | 1349-7006 1347-9032 |
| DOI: | 10.1111/j.1349-7006.2007.00422.x |
| Popis: | Small ubiquitin-related modifier (SUMO) modification appears to regulate the activity, intracellular localization, and stability of the targeted proteins. To explore the relationship among sumoylation, antitumor reagent, and apoptosis, we treated green fluorescence protein (GFP)-SUMO-1-overexpressed K562 cells (K562/GFP-SUMO-1) with mitoxantrone (MIT) as an antitumor reagent. By the treatment with MIT, GFP-SUMO-1 formed foci in nuclei. While by the treatment with a tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), GFP-SUMO-1 located homogeneously in nuclei. When K562/GFP-SUMO-1 cells were treated with TPA plus MIT, GFP-SUMO-1 foci became larger and apoptosis was induced more than with MIT alone. The apoptosis induced by TPA plus MIT was prevented by blockage of GFP-SUMO-1 foci by small interfering RNA (siRNA) against SUMO-1. The formation of GFP-SUMO-1 foci was reduced by a MEK inhibitor U0126 or a nuclear export inhibitor leptomycin B, and endogenous SUMO-1 foci were reduced in K562 cells expressing the dominant-negative MEK1 mutant. These results suggest that the formation of SUMO-1 foci is regulated by the MEK-ERK pathway and may induce apoptosis. |
| Databáze: | OpenAIRE |
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