Remodeling of the infection chamber before infection thread formation reveals a two-step mechanism for rhizobial entry into the host legume root hair
| Autor: | David G. Barker, Erik Limpens, Joëlle Fournier, Mireille Chabaud, Sergey Ivanov, Andrea Genre, Alice Teillet, Fernanda de Carvalho-Niebel |
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| Přispěvatelé: | Laboratoire des interactions plantes micro-organismes (LIPM), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Wageningen University and Research Centre (WUR), Universita di Torino, French National Research Agency [ANR-08-BLAN-0029-01], Partenariat Hubert Curien Galilee [30111WJ], French National Laboratoire d'Excellence TULIP initiative [ANR-10-LABX-41], Wageningen University and Research [Wageningen] (WUR), University of Turin, The Partenariat Hubert Curien Galilee (grant no. 30111WJ to A.G. and D.G.B.), ANR-08-BLAN-0029,SYMDYNAMICS,Mécanismes d'accommodation endosymbiotique chez les végétaux: Dynamique intracellulaire et signalisation calcique(2008), ANR-10-LABX-0041,TULIP,Towards a Unified theory of biotic Interactions: the roLe of environmental(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
Physiology [SDV]Life Sciences [q-bio] Mutant Plant Science Root hair Exocytosis Rhizobia Cell wall Symbiosis Botany Genetics Laboratorium voor Moleculaire Biologie [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Life Science Sinorhizobium meliloti EPS-1 biology fungi food and beverages [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics biology.organism_classification Medicago truncatula Cell biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Laboratory of Molecular Biology |
| Zdroj: | Plant Physiology 167 (2015) 4 Plant Physiology Plant Physiology, American Society of Plant Biologists, 2015, 167 (4), pp.1233-1242. ⟨10.1104/pp.114.253302⟩ Plant Physiology, 167(4), 1233-1242 |
| ISSN: | 0032-0889 1532-2548 |
| DOI: | 10.1104/pp.114.253302⟩ |
| Popis: | International audience; In many legumes, root entry of symbiotic nitrogen-fixing rhizobia occurs via host-constructed tubular tip-growing structures known as infection threads (ITs). Here, we have used a confocal microscopy live-tissue imaging approach to investigate early stages of IT formation in Medicago truncatula root hairs (RHs) expressing fluorescent protein fusion reporters. This has revealed that ITs only initiate 10 to 20 h after the completion of RH curling, by which time major modifications have occurred within the so-called infection chamber, the site of bacterial entrapment. These include the accumulation of exocytosis (M. truncatula Vesicle-Associated Membrane Protein721e)- and cell wall (M. truncatula EARLY NODULIN11)-associated markers, concomitant with radial expansion of the chamber. Significantly, the infection-defective M. truncatula nodule inception-1 mutant is unable to create a functional infection chamber. This underlines the importance of the NIN-dependent phase of host cell wall remodeling that accompanies bacterial proliferation and precedes IT formation, and leads us to propose a two-step model for rhizobial infection initiation in legume RHs. |
| Databáze: | OpenAIRE |
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