Transcriptomic view of detached lettuce leaves during storage: A crosstalk between wounding, dehydration and senescence
| Autor: | David Roux, Christophe Klopp, Sandrine Laurent, Félicie Lauri, Florence Charles, Julie Ripoll, Huguette Sallanon, Véronique Vidal |
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| Přispěvatelé: | Unité plantes et systèmes de culture horticoles, Institut National de la Recherche Agronomique (INRA), Centre de Recherche sur l'Education, les apprentissages et la didactique (CREAD), Institut universitaire de formation des maîtres - Bretagne (IUFM Bretagne), Université de Brest (UBO)-Université de Brest (UBO)-Université européenne de Bretagne - European University of Brittany (UEB)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Laboratoire de Chimie, Catalyse, Polymères et Procédés, R 5265 (C2P2), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Unité de Biométrie et Intelligence Artificielle (UBIA), Avignon Université (AU), Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Unité de recherche Plantes et Systèmes de Culture Horticoles (PSH), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de La Réunion (UR)-Université de Montpellier (UM)-Avignon Université (AU)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Senescence [SDV]Life Sciences [q-bio] Cold storage Storage Horticulture Biology 01 natural sciences 040501 horticulture Transcriptome chemistry.chemical_compound Lactuca sativa L Gene expression Postharvest [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Abscisic acid Chlorophyll fluorescence ComputingMilieux_MISCELLANEOUS 04 agricultural and veterinary sciences Cell biology chemistry Chlorophyll Wounding 0405 other agricultural sciences Agronomy and Crop Science 010606 plant biology & botany Food Science |
| Zdroj: | Postharvest Biology and Technology Postharvest Biology and Technology, Elsevier, 2019, 152, pp.73-88. ⟨10.1016/j.postharvbio.2019.02.004⟩ |
| ISSN: | 0925-5214 |
| Popis: | International audience; Many methods of storage are currently used to delay the postharvest senescence and the ensuing quality losses of detached lettuce leaves. This transcriptome study explores the senescence mechanisms of packaged lettuce leaves over 7 d of cold storage in darkness. At the end of the storage, the detached lettuce leaves showed a 10% water loss and 24% cut-edge discoloration. No chlorophyll fluorescence variation was observed. A total of 1048 and 1846 genes were differentially expressed (DE) after 2 and 7 d of storage, respectively. In terms of gene expression modulation, the data showed a global shutdown of primary-metabolism but no obvious chlorophyll breakdown. Although an early stress-response clearly takes place, the cold storage under darkness appeared to delay senescence by protecting chloroplasts from degradation and enhancing an antioxidative response. Then, a progressive global expression shutdown appeared, concomitantly with the dehydration stress. Among the transcripts that showed an early high expression, the data highlighted many stress-related genes (PIN, bzip TF, Heat-shock, stress A-like), 30 redox-regulation associated transcripts, the majority of the phenylpropanoid pathway markers and ethylene and auxin-related genes. Later (day 7), many dehydration-responsive markers showed an increased expression, as did some abscisic acid related markers. Phytohormones (cytokinins) and transcriptions factors (WRKYs) appeared to play complex roles as senescence progressed. No simple link between cut-edge discoloration and PPO-related genes expression was made. The main known process of senescence induction through the phaephorbide a oxygenase and phospholipase D pathways were not highlighted in this study. To the best of our knowledge, this study is the first to explore the transcriptional response of postharvest senescence in detached lettuce leaves. Deeper analysis of several markers will help to dissect the crosstalk between wounding, dehydration and senescence mechanisms. In addition, post-transcriptional studies are needed to conclude about the described patterns. |
| Databáze: | OpenAIRE |
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