Vaccination against type F botulinum toxin using attenuated Salmonella enterica var Typhimurium strains expressing the BoNT/F HC fragment

Autor: Jane L. Holley, Susan Foynes, Richard W. Titball, Neil F. Fairweather, Helen S. Garmory
Rok vydání: 2003
Předmět:
Salmonella typhimurium
Salmonella
Botulinum Toxins
Genetic Vectors
Administration
Oral
Vaccines
Attenuated
medicine.disease_cause
Microbiology
law.invention
Mice
Bacterial Proteins
law
Clostridium botulinum
Genes
Synthetic
medicine
Animals
Botulism
Promoter Regions
Genetic
Heat-Shock Proteins
Mice
Inbred BALB C
Vaccines
Synthetic
General Veterinary
General Immunology and Microbiology
biology
Serine Endopeptidases
Vaccination
Public Health
Environmental and Occupational Health
Membrane Proteins
Gene Expression Regulation
Bacterial
biology.organism_classification
medicine.disease
Antibodies
Bacterial
Virology
Enterobacteriaceae
Botulinum toxin
Peptide Fragments
Infectious Diseases
Salmonella enterica
Bacterial Vaccines
Recombinant DNA
Feasibility Studies
Molecular Medicine
Female
Periplasmic Proteins
medicine.drug
Zdroj: Vaccine. 21:1052-1059
ISSN: 0264-410X
DOI: 10.1016/s0264-410x(02)00622-9
Popis: The utility of the htrA , pagC and nirB promoters to direct the expression of the carboxy-terminal (H C ) fragment of botulinum toxin F (FH C ) in Salmonella enterica var Typhimurium has been evaluated. Only low levels of serum antibody were induced after immunisation, and some protection against botulinum toxin type F was demonstrated after oral immunisation of mice with two doses of any of these recombinant Salmonella . Immunisation with two doses of recombinant Salmonella expressing FH C from the htrA promoter gave the greatest protection, against up to 10,000 mouse lethal doses of botulinum toxin type F. These results demonstrate the feasibility of an orally delivered vaccine against botulinum toxin type F.
Databáze: OpenAIRE
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