Characterization of the heparin/heparan sulfate binding site of the natural cytotoxicity receptor NKp46
| Autor: | Eyal Fima, Toin H van Kuppevelt, Ahuva Bar-Ilan, Oren Hershkovitz, Sharona Elgavish, J. T. Gallagher, Noga Bloushtain, Fabrice Benchetrit, Guy Landau, Alon Zilka, Angel Porgador |
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| Rok vydání: | 2005 |
| Předmět: |
Models
Molecular Molecular Sequence Data Biochemistry Epitope Protein Structure Secondary chemistry.chemical_compound Cell Line Tumor medicine Humans Amino Acid Sequence Binding site Receptors Immunologic Renal disorder [IGMD 9] chemistry.chemical_classification Binding Sites Membrane Glycoproteins biology Heparin Natural Cytotoxicity Triggering Receptor 1 Anticoagulants Heparan sulfate Tissue engineering and pathology [NCMLS 3] Amino acid Protein Structure Tertiary Fibronectin Tumor microenvironment [UMCN 1.3] chemistry Mutation biology.protein Heparan sulfate binding Heparitin Sulfate Sequence Alignment medicine.drug Protein Binding |
| Zdroj: | Biochemistry, 44, 44, pp. 14477-85 Biochemistry, 44, 14477-85 |
| ISSN: | 0006-2960 |
| Popis: | Contains fulltext : 48635.pdf (Publisher’s version ) (Closed access) NKp46 is a member of a group of receptors collectively termed natural cytotoxicity receptors (NCRs) that are expressed by natural killer (NK) cells. NCRs are capable of mediating direct killing of tumor and virus-infected cells by NK cells. We have recently shown that NKp46 recognizes the heparan sulfate moieties of membranal heparan sulfate proteoglycans (HSPGs), thus enabling lysis of tumor cells by NK cells. In the current study, we further examined the residues in NKp46 that may be involved in heparan sulfate binding on tumor cells. On the basis of both the electrostatic potential map and comparison to the heparin binding site on human fibronectin, we predicted a continuous region containing the basic amino acids K133, R136, H139, R142, and K146 to be involved in NKp46 binding to heparan sulfate. Mutating these amino acids on NKp46D2 to noncharged amino acids retained its virus binding capacity but reduced its binding to tumor cells with a 10-100 fold lower K(D) when tested for direct binding to heparin. The minimal length of the heparin/heparan sulfate epitope recognized by NKp46 was eight saccharides as predicted from the structure and proven by testing heparin oligomers. Testing selectively monodesulfated heparin oligomers emphasized the specific contributions of O-sulfation, N-sulfation, and N-acetylation to epitope recognition by NKp46. The characterization of heparan sulfate binding region in NKp46 offers further insight into the identity of the ligands for NKp46 and the interaction of NK and cancers. |
| Databáze: | OpenAIRE |
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