Distribution of plasminogen activator in different fractions of bovine milk
| Autor: | Ming R. Guo, Ioannis Politis, Jeffrey H. White, Paul S. Kindstedt, James A. Gilmore, Kristen O'Hare, B. Zavizion |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
food.ingredient
Somatic cell Amiloride Plasminogen Activators fluids and secretions food Casein Skimmed milk medicine Animals Micelles Fibrin Molecular mass T-plasminogen activator Chemistry Caseins food and beverages General Medicine Hydrogen-Ion Concentration Raw milk Urokinase-Type Plasminogen Activator Molecular Weight Milk Biochemistry Tissue Plasminogen Activator Cattle Electrophoresis Polyacrylamide Gel Female Animal Science and Zoology Plasminogen activator Food Science medicine.drug |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1469-7629 0022-0299 |
| DOI: | 10.1017/s0022029900033720 |
| Popis: | SUMMARYThe type and relative amounts of plasminogen activator (PA) in different fractions of bovine milk obtained from 15 Holstein cows were examined. Raw milk was centrifuged to separate skim milk and a somatic cell pellet. PA was mainly localized within the casein fraction, being 42 times that in the serum, and in association with somatic cells. The predominant form of PA in milk casein was isolated from SDS-PAGE gel extracts and had a molecular mass of ∽75 kDa. Its activity was increased 41-fold (P < 0·01) in the presence of fibrin but was unaffected by the presence of amiloride, indicating that it was due to tissue-PA. The predominant forms of PA associated with milk somatic cells were isolated from SDS-PAGE gel extracts and had molecular masses of ∽ 30 and ∽ 50 kDa. The activity of both proteins was unaffected by the presence of fibrin but was dramatically reduced by the presence of amiloride, indicating that they represented urokinase-PA. |
| Databáze: | OpenAIRE |
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