Optimal phenotypic testing of AmpC beta-lactamases using boronic acid solutions
| Autor: | John Merlino, Thomas Gottlieb, Wei-Yuen Su |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Klebsiella pneumoniae Microbial Sensitivity Tests Biology medicine.disease_cause beta-Lactamases Microbiology law.invention chemistry.chemical_compound Plasmid Bacterial Proteins law Escherichia coli medicine Phenylboronic acid Polymerase chain reaction General Medicine biology.organism_classification Boronic Acids Phenotype Enterobacteriaceae Anti-Bacterial Agents Infectious Diseases Biochemistry chemistry Boronic acid Plasmids |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 31:49-51 |
| ISSN: | 1435-4373 0934-9723 |
| DOI: | 10.1007/s10096-011-1271-5 |
| Popis: | Plasmid-mediated class C β-lactamases are reported from Escherichia coli and Klebsiella pneumoniae with increasing frequency. No screening and confirmatory tests have been uniformly established for these strains. We investigated for the presence of plasmid-mediated AmpC production in 51 clinical isolates of Enterobacteriaceae, comparing two different boronic acid formulations, phenylboronic acid (PB) and 3-(N-Boc-amino)phenylboronic acid (APB), using polymerase chain reaction (PCR). PB performed better than APB. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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