Mas37p, a novel receptor subunit for protein import into mitochondria
| Autor: | Sepp D. Kohlwein, Ronald Bauer, Martin Horst, Erwin Lamping, Gottfried Schatz, Fritz Paltauf, Trevor Lithgow, Tina Junne, Volker Haucke, Sabine Gratzer |
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| Rok vydání: | 1995 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Macromolecular Substances Protein Conformation Genes Fungal Molecular Sequence Data Saccharomyces cerevisiae Mutant Gene Expression TIM/TOM complex Sulfur Radioisotopes Cytosol Protein structure Amino Acid Sequence Cloning Molecular Receptor Peptide sequence Sorting and assembly machinery Base Sequence biology Sulfates Temperature Membrane Proteins Articles Cell Biology biology.organism_classification Mitochondria Models Structural Biochemistry Mutation Bacterial outer membrane |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | By screening a collection of Saccharomyces cerevisiae mutants temperature sensitive for growth on a nonfermentable carbon source, we have isolated a gene (termed MAS37) which encodes a novel receptor for protein import into mitochondria. Mas37p is a 37-kD outer membrane protein with two putative membrane-spanning regions. Inactivation of the MAS37 gene renders cells temperature-sensitive for respiration-driven growth, inhibits import of precursors into isolated mitochondria, and is synthetically lethal with a deletion of one of the genes encoding the import receptors Mas70p or Mas20p. Inactivation of Mas37p with specific antibodies inhibits import of different precursors to different extents; the precursor specificity of Mas37p resembles that of the previously described import receptor Mas70p. Mas70p and Mas37p form a 1:1 complex in detergent extracts of mitochondria and overexpression of one protein enhances that of the other. We suggest that the Mas37p/Mas70p heterodimer functions as a receptor for protein import into yeast mitochondria and that the mitochondrial receptor system consists of hetero-oligomeric subcomplexes with distinct binding activities, but overlapping precursor specificities. |
| Databáze: | OpenAIRE |
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