Comparison of Donor Cornea Endothelial Cell Density Determined by Eye Banks and by a Central Reading Center in the Cornea Preservation Time Study
| Autor: | Jonathan H. Lass, Christopher G. Stoeger, Jameson Clover, Kristen McCoy, Pankaj C Gupta, Allison R Ayala, Peter Bedard, Sanjay V. Patel, Jonathan C. Song, Beth Ann Benetz, Loretta B Szczotka-Flynn, Robert C OʼBrien, Harry J. Menegay, Maureen G. Maguire |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent genetic structures Endothelium Cell Count Eye Banks Article Young Adult Ophthalmology Cornea Image Processing Computer-Assisted medicine Humans Donor cornea Child Aged Chemistry Endothelium Corneal Corneal Endothelial Cell Loss Middle Aged Descemet stripping automated endothelial keratoplasty Endothelial cell density medicine.anatomical_structure SPECULAR MICROSCOPY Descemet Stripping Endothelial Keratoplasty Female DONOR EVALUATION |
| Zdroj: | Cornea |
| ISSN: | 0277-3740 |
| DOI: | 10.1097/ico.0000000000001846 |
| Popis: | PURPOSE: To evaluate agreement between eye banks (EBs) and a reading center on endothelial cell density (ECD) determinations in the Cornea Preservation Time Study. METHODS: The Cornea Image Analysis Reading Center (CIARC) performed variable frame image analysis on EB-obtained–preoperative central endothelial images (after lamellar dissection for Descemet stripping automated endothelial keratoplasty by the EBs or before shipping, if surgeon prepared) to determine ECD. The EBs performed their usual method of ECD determination. The CIARC and EBs also provided ECD determinations from screening central endothelial images taken by the EBs during donor evaluation. Two independent masked CIARC readers determined ECD with measurements averaged. RESULTS: The mean preoperative ECD was 15 cells/mm(2) greater by the EBs than by CIARC (N = 1286, P < 0.001) with 95% limits of agreement of (−644, 675 cells/mm(2)). The limits of agreement in preoperative ECD were wider in the After-Lamellar-Dissection Group (−687, 683 cells/mm(2)) than in the Before Shipping Group [(−505, 633 cells/mm(2)); P = 0.03]. The EBs-determined preoperative ECD was within 10% of the CIARC-determined ECD for 886 (69%) image sets, with 236 (18%) higher by >10% and 164 (13%) lower by >10%. Excellent agreement appeared between the EBs and CIARC when 100–300 cells could be analyzed in contrast to |
| Databáze: | OpenAIRE |
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