Molecular alterations in a new cell line (KU-Lu-MPPt3) established from a human lung adenocarcinoma with a micropapillary pattern
| Autor: | Yoshio Matsui, Yukitoshi Satoh, Masahito Naito, Dai Sonoda, Tsutomu Yoshida, Kazu Shiomi, Masashi Mikubo, Yukiko Matsuo |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology Cancer Research medicine.medical_specialty Pathology Lung Neoplasms Mice Nude Adenocarcinoma of Lung Vimentin Mice SCID Adenocarcinoma Mice 03 medical and health sciences Cytokeratin 0302 clinical medicine Mice Inbred NOD Cell Line Tumor Internal medicine medicine Animals Humans Epidermal growth factor receptor Lymph node Aged biology business.industry Cancer Genes erbB-1 General Medicine medicine.disease Immunohistochemistry Primary tumor ErbB Receptors 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Mediastinal lymph node Mutation biology.protein Heterografts Female business |
| Zdroj: | Journal of Cancer Research and Clinical Oncology. 144:75-87 |
| ISSN: | 1432-1335 0171-5216 |
| Popis: | Lung adenocarcinomas with a micropapillary pattern (MPP) are characterized by more frequent and pronounced vascular invasion, higher incidence and more advanced lymph node involvement and poorer prognosis than papillary adenocarcinomas without an MPP. Here we established a new lung cancer cell line featuring micropapillary structure. A 73-year-old never-smoker Japanese female, presenting with an abnormal chest shadow, was diagnosed with a clinical T2aN0M0 Stage IB lung adenocarcinoma and underwent left upper lobectomy with mediastinal lymph node dissection. Pathological study demonstrated a T2aN2M0 Stage IIIA micropapillary adenocarcinoma. Tumor cells were obtained from freshly resected lung material and used to establish the KU-Lu-MPPt3 cell line. The KU-Lu-MPPt3 cells featured adherent monolayers, adherent tufts, and suspended tufts without adhesion under the same culture conditions. The cells were positive for cytokeratin, epithelial cell-adhesion molecules, E-cadherin, mucin-1, thyroid transcription factor-1, vimentin, and anti-programmed death ligand 1. Xenograft tumors clearly demonstrated micropapillary structures. Sequencing and fragment analysis of the epidermal growth factor receptor in the primary tumor tissue and KU-Lu-MPPt3 cells revealed an in-frame deletion E746-A750 in exon 19. This cell line represents a new model system for molecular studies of lung adenocarcinoma which may be suitable for investigation of cancer spread and also for development of molecular-targeting and immunotherapies, both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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