Isolation of normal immunoglobulin E by means of an immunoadsorbent
| Autor: | Lore Theilkäs, Silvio Barandun, F. Skvaril, S. Carrel, Andreas Morell |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Electrophoresis
Immunodiffusion History Swine Black People Immunoglobulins Improved method Immunoglobulin E White People Immunoglobulin G Education Animals Humans Polyacrylamide gel electrophoresis Serum pool Antiserum Acrylamides Sheep biology Chemistry Immune Sera Dextrans Articles Chromatography Ion Exchange Virology Molecular biology Computer Science Applications Acrylates Immunoglobulin M biology.protein Adsorption Rabbits Healthy donor Antibody Multiple Myeloma Bence Jones Protein |
| Zdroj: | Biochemical Journal. 122:405-408 |
| ISSN: | 0306-3283 |
| Popis: | An improved method for the isolation of normal immunoglobulin E is described. The method is based on the use of an immunoadsorbent formed by the mechanical entrapment of antibodies against a myeloma immunoglobulin E into a lattice of a highly cross-linked macroporous polyacrylamide gel. Normal immunoglobulin E was isolated from an immunoglobulin E-rich serum pool as well as from an individual healthy donor. The isolated immunoglobulin E was contaminated with about 20% of immunoglobulin G. An antiserum against normal immunoglobulin E was prepared by immunizing rabbits with the isolated immunoglobulin E. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|