Matrix metalloproteinase (MMP) induction and inhibition at different doses of recombinant tissue plasminogen activator following experimental stroke
| Autor: | Helge K. Martens, Gerhard F. Hamann, Martin Dichgans, Dorothe Burggraf |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Collagen Type IV
medicine.medical_specialty Pathology Proteases Biology Matrix metalloproteinase Matrix Metalloproteinase Inhibitors Brain ischemia Internal medicine medicine Animals Inducer Zymography Tissue Inhibitor of Metalloproteinase-2 Tissue Inhibitor of Metalloproteinase-1 Dose-Response Relationship Drug T-plasminogen activator Hematology medicine.disease Matrix Metalloproteinases Rats Stroke Disease Models Animal Endocrinology medicine.anatomical_structure Treatment Outcome Gene Expression Regulation Matrix Metalloproteinase 9 Tissue Plasminogen Activator Basigin Interstitial collagenase Matrix Metalloproteinase 2 Basal lamina |
| Zdroj: | Thrombosis and haemostasis. 98(5) |
| ISSN: | 0340-6245 |
| Popis: | SummaryAlthough recombinant tissue plasminogen activator (rt-PA) is successfully used for thrombolysis in human stroke, it may increase the risk of haemorrhagic complications. It was shown that the matrix metalloproteinase (MMP) system is critically involved in basal lamina degradation after middle cerebral artery occlusion and reperfusion following rt-PA administration. We describe the effects of different doses of rt-PA (saline, 0.9, 9, or 18 mg rt-PA/kg body weight) on the MMPs, their specific inhibitors (TIMPs), and also their inducer protein EMMPRIN following experimental cerebral ischemia (3 hours [h], 24 h reperfusion, suture model) in rats. The amount of MMP-2 and -9 was measured by gelatine zymography, TIMP-1 and -2 by reverse gelatine zymography, and the content of EMMPRIN and the basal lamina component collagen type IV by Western blotting. The amount of both MMPs steadily rose with increasing doses of rt- PA (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|